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Validation Data Gallery

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  • Western Blot (WB) analysis of rat brain tissue using CHAT Polyclonal antibody (24418-1-AP)

    WB analysis of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using CHAT Polyclonal antibody (24418-1-AP)

    WB analysis of human placenta using 24418-1-AP (same clone as 24418-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CHAT Polyclonal antibody (24418-1-AP)

    WB analysis of mouse brain using 24418-1-AP (same clone as 24418-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat brain tissue using CHAT Polyclonal antibody (24418-1-AP)

    IP experiment of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

    IP result of anti-CHAT (IP:24418-1-AP, 4ug; Detection:24418-1-AP 1:2000) with rat brain tissue lysate 1880 ug. This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using CHAT Polyclonal antibody (24418-1-AP)

    IHC staining of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using CHAT Polyclonal antibody (24418-1-AP)

    IHC staining of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using CHAT Polyclonal antibody (24418-1-AP)

    IHC staining of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHAT Polyclonal antibody (24418-1-AP)

    IHC staining of mouse brain using 24418-1-AP (same clone as 24418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24418-1-AP (CHAT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHAT Polyclonal antibody (24418-1-AP)

    IHC staining of mouse brain using 24418-1-AP (same clone as 24418-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24418-1-AP (CHAT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CHAT Polyclonal antibody (24418-1-AP)

    IF Staining of mouse brain using 24418-1-AP (same clone as 24418-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 24418-1-AP (CHAT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

CHAT Polyclonal antibody, PBS Only

CHAT Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 24418-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24418-1-PBS targets CHAT in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag19550

Product name: Recombinant human CHAT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 281-630 aa of BC130617

Sequence: SDTHRALQLLHGGGYSKNGANRWYDKSLQFVVGRDGTCGVVCEHSPFDGIVLVQCTEHLLKHMTQSSRKLIRADSVSELPAPRRLRWKCSPEIQGHLASSAEKLQRIVKNLDFIVYKFDNYGKTFIKKQKCSPDAFIQVALQLAFYRLHRRLVPTYESASIRRFQEGRVDNIRSATPEALAFVRAVTDHKAAVPASEKLLLLKDAIRAQTAYTVMAITGMAIDNHLLALRELARAMCKELPEMFMDETYLMSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

相同性解析による交差性が予測される生物種
Full Name choline acetyltransferase
Calculated molecular weight 748 aa, 83 kDa
Observed molecular weight 60-66 kDa, 45 kDa
GenBank accession numberBC130617
Gene Symbol CHAT
Gene ID (NCBI) 1103
RRIDAB_2879536
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP28329
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. It has been observed that CHAT gene generates multiple mRNA splice variants, including peripheral type CHAT (pCHAT) and common type CHAT (cCHAT), encoding 40-55 kDa and 66-70 kDa forms of proteins, respectively.

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