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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHAT Polyclonal antibody (20747-1-AP)

    WB analysis using 20747-1-AP (same clone as 20747-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20747-1-AP (CHAT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse placenta tissue using CHAT Polyclonal antibody (20747-1-AP)

    WB analysis of mouse placenta using 20747-1-AP (same clone as 20747-1-PBS)

    mouse placenta tissue were subjected to SDS PAGE followed by western blot with 20747-1-AP (CHAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat brain tissue using CHAT Polyclonal antibody (20747-1-AP)

    IP experiment of rat brain using 20747-1-AP (same clone as 20747-1-PBS)

    IP result of anti-CHAT (IP:20747-1-AP, 4ug; Detection:20747-1-AP 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using CHAT Polyclonal antibody (20747-1-AP)

    IHC staining of rat brain using 20747-1-AP (same clone as 20747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHAT Polyclonal antibody (20747-1-AP)

    IHC staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHAT Polyclonal antibody (20747-1-AP)

    IHC staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CHAT Polyclonal antibody (20747-1-AP)

    IHC staining of human lung cancer using 20747-1-AP (same clone as 20747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CHAT Polyclonal antibody (20747-1-AP)

    IHC staining of human lung cancer using 20747-1-AP (same clone as 20747-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CHAT Polyclonal antibody (20747-1-AP)

    IF Staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CHAT antibody (20747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using CHAT Polyclonal antibody (20747-1-AP)

    IF Staining of Neuro-2a using 20747-1-AP (same clone as 20747-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CHAT antibody (20747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

CHAT Polyclonal antibody, PBS Only

CHAT Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 20747-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Synonyms

CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, EC:2.3.1.6

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20747-1-PBS targets CHAT in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name choline acetyltransferase
Calculated molecular weight 83 kDa
Observed molecular weight 47-50 kDa, 66-70 kDa
GenBank accession numberNM_020549
Gene Symbol CHAT
Gene ID (NCBI) 1103
RRIDAB_10898169
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP28329
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. Human CHAT gene generates multiple mRNA splice variants (PMID: 10861222). All these variants possess start codons for translation of 70 kDa cChAT protein (isoform 1). The M variant has an additional start codon for 83 kDa cChAT protein (isoform 2), which has been found in nuclei of neuronal cells in human CNS; and the S variant has another codon for 74 kDa cChAT protein (isoform 3). Besides, CHAT includes a short exon-skipping splice variant (peripheral type CHAT) pCHAT encoding 40-55 kDa (PMID: 31040770, 29759072). This antibody can recognize both pCHAT and cCHAT.

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