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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CHAD Polyclonal antibody (12963-1-AP)

    WB analysis of HeLa using 12963-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12963-1-AP (CHAD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using CHAD Polyclonal antibody (12963-1-AP)

    WB analysis of MCF-7 using 12963-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12963-1-AP (CHAD antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CHAD Polyclonal antibody (12963-1-AP)

    WB analysis of Jurkat using 12963-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12963-1-AP (CHAD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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CHAD Polyclonal antibody

CHAD Polyclonal Antibody for WB, ELISA
Cat No. 12963-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

Cartilage leucine rich protein, CHAD, chondroadherin, SLRR4A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12963-1-AP targets CHAD in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHAD fusion protein Ag4077 相同性解析による交差性が予測される生物種
Full Name chondroadherin
Calculated molecular weight 359 aa, 40.5 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC036360
Gene Symbol CHAD
Gene ID (NCBI) 1101
RRIDAB_2244851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15335
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Chondroadherin (CHAD), also known as cartilage leucine-rich protein, is a prominent noncollagenous extracellular protein in cartilage. It was first isolated from bovine cartilage. The primary structure of CHAD shows that it belongs to the family of leucine-rich repeat (LRR) proteins. It is expressed at high levels in certain zones of cartilage and also has been detected in bone, tendon, bone marrow, and chondrosarcoma cells. CHAD promotes attachment of chondrocytes, fibroblasts, and osteoblasts, mediated via the integrin alpha2beta1. It has also been shown to bind to collagen type II and both collagen type II and chondroadherin interact with integrin alpha2beta1. Besides, CHAD may play an important role in the regulation of chondrocyte growth and proliferation.

Protocols

Product Specific Protocols
WB protocol for CHAD antibody 12963-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Reprod Sci

NFAT Overexpression Correlates with CA72-4 and Poor Prognosis of Ovarian Clear-Cell Carcinoma Subtype.

Authors - Bing Xin
View Article