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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CGGBP1 Polyclonal antibody (10716-1-AP)

    WB analysis using 10716-1-AP (same clone as 10716-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10716-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using CGGBP1 Polyclonal antibody (10716-1-AP)

    WB analysis of mouse thymus using 10716-1-AP (same clone as 10716-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10716-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using CGGBP1 Polyclonal antibody (10716-1-AP)

    WB analysis of K-562 using 10716-1-AP (same clone as 10716-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10716-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using CGGBP1 Polyclonal antibody (10716-1-AP)

    WB analysis of Raji using 10716-1-AP (same clone as 10716-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10716-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using CGGBP1 Polyclonal antibody (10716-1-AP)

    WB analysis of HL-60 using 10716-1-AP (same clone as 10716-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 10716-1-AP (CGGBP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10716-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CGGBP1 Polyclonal antibody (10716-1-AP)

    IHC staining of human breast cancer using 10716-1-AP (same clone as 10716-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10716-1-AP (CGGBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10716-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CGGBP1 Polyclonal antibody, PBS Only

CGGBP1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10716-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10716-1-PBS targets CGGBP1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1129

Product name: Recombinant human CGGBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-167 aa of BC005222

Sequence: MERFVVTAPPARNRSKTALYVTPLDRVTEFGGELHEDGGKLFCTSCNVVLNHVRKSAISDHLKSKTHTKRKAEFEEQNVRKKQRPLTASLQCNSTAQTEKVSVIQDFVKMCLEANIPLEKADHPAVRAFLSRHVKNGGSIPKSDQLRRTYLPDGYENENQLLNSQDC

相同性解析による交差性が予測される生物種
Full Name CGG triplet repeat binding protein 1
Calculated molecular weight 19 kDa
Observed molecular weight 19-23 kDa
GenBank accession numberBC005222
Gene Symbol CGGBP1
Gene ID (NCBI) 8545
RRIDAB_2079283
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UFW8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CGGBP1, also named as CGGBP, is a 20 kDa CGG-binding protein. It binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter. CGGBP1 may play a role in regulating FMR1 promoter. It is a bona fide midbody protein required for normal abscission and mitosis in general.(PMID:20832400)

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