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Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using CFP Recombinant antibody (85999-1-RR)

    WB analysis of human plasma using 85999-1-RR (same clone as 85999-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 85999-1-RR (CFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85999-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CFP Recombinant antibody (85999-1-RR)

    WB analysis using 85999-1-RR (same clone as 85999-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85999-1-RR (CFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85999-1-PBS in a different storage buffer formulation.

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CFP Recombinant antibody, PBS Only

CFP Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85999-1-PBS
Clone No.250654E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

BFD, Complement factor P, complement factor properdin, PFC

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85999-1-PBS targets CFP in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CFP fusion protein Eg2219 相同性解析による交差性が予測される生物種
Full Name complement factor properdin
Calculated molecular weight 469 aa, 51 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC015756
Gene Symbol CFP
Gene ID (NCBI) 5199
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP27918
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CFP, also named as Properdin or PFC, is a 469 amino acid protein, which contains 7 TSP type-1 domains. CFP as secreted protein is a positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Properdin deficiency poses a significant risk for severe meningococcal infections (PMID: 22229731). Properdin may be novel biomarker for future risk of type 2 diabetes (PMID: 22338105).