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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Properdin/PFC Polyclonal antibody (17192-1-AP)

    WB analysis of Jurkat using 17192-1-AP (same clone as 17192-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17192-1-AP (CFP Antibody) at dilution of 1:300 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 17192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using Properdin/PFC Polyclonal antibody (17192-1-AP)

    IHC staining of human spleen using 17192-1-AP (same clone as 17192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17192-1-AP (CFP Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17192-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using Properdin/PFC Polyclonal antibody (17192-1-AP)

    IHC staining of human spleen using 17192-1-AP (same clone as 17192-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 17192-1-AP (CFP Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17192-1-PBS in a different storage buffer formulation.

Properdin/PFC Polyclonal antibody, PBS Only

Properdin/PFC Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17192-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

CFP, BFD, Complement factor P, complement factor properdin, PFC

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17192-1-PBS targets Properdin/PFC in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9798

Product name: Recombinant human CFP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 120-469 aa of BC015756

Sequence: LEWQLQACEDQQCCPEMGGWSGWGPWEPCSVTCSKGTRTRRRACNHPAPKCGGHCPGQAQESEACDTQQVCPTHGAWATWGPWTPCSASCHGGPHEPKETRSRKCSAPEPSQKPPGKPCPGLAYEQRRCTGLPPCPVAGGWGPWGPVSPCPVTCGLGQTMEQRTCNHPVPQHGGPFCAGDATRTHICNTAVPCPVDGEWDSWGEWSPCIRRNMKSISCQEIPGQQSRGRTCRGRKFDGHRCAGQQQDIRHCYSIQHCPLKGSWSEWSTWGLCMPPCGPNPTRARQRLCTPLLPKYPPTVSMVEGQGEKNVTFWGRPLPRCEELQGQKLVVEEKRPCLHVPACKDPEEEEL

相同性解析による交差性が予測される生物種
Full Name complement factor properdin
Calculated molecular weight 469 aa, 51 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC015756
Gene Symbol CFP
Gene ID (NCBI) 5199
RRIDAB_2878359
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP27918
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CFP, also named as Properdin or PFC, is a 469 amino acid protein, which contains 7 TSP type-1 domains. CFP as secreted protein is a positive regulator of the alternate pathway of complement. It binds to and stabilizes the C3- and C5-convertase enzyme complexes. Properdin deficiency poses a significant risk for severe meningococcal infections (PMID: 22229731). Properdin may be novel biomarker for future risk of type 2 diabetes (PMID: 22338105).