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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CFLAR/FLIP Polyclonal antibody (10394-1-AP)

    WB analysis of Jurkat using 10394-1-AP (same clone as 10394-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CFLAR/FLIP Polyclonal antibody (10394-1-AP)

    WB analysis of HepG2 using 10394-1-AP (same clone as 10394-1-PBS)

    WB result of CFLAR/FLIP antibody (10394-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-CFLAR/FLIP transfected HepG2 cells. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CFLAR/FLIP Polyclonal antibody (10394-1-AP)

    WB analysis of HeLa using 10394-1-AP (same clone as 10394-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using CFLAR/FLIP Polyclonal antibody (10394-1-AP)

    IP experiment of Jurkat using 10394-1-AP (same clone as 10394-1-PBS)

    IP result of anti-CFLAR/FLIP (IP:10394-1-AP, 4ug; Detection:10394-1-AP 1:500) with Jurkat cells lysate 4000ug. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using CFLAR/FLIP Polyclonal antibody (10394-1-AP)

    IP experiment of Jurkat using 10394-1-AP (same clone as 10394-1-PBS)

    IP result of anti-CFLAR/FLIP (IP:10394-1-AP, 4ug; Detection:10394-1-AP 1:500) with Jurkat cells lysate 4000ug. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using CFLAR/FLIP Polyclonal antibody (10394-1-AP)

    IHC staining of human skeletal muscle using 10394-1-AP (same clone as 10394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 10394-1-AP (CFLAR/FLIP antibody at dilution of 1:50. This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CFLAR/FLIP Polyclonal antibody (10394-1-AP)

    IHC staining of mouse skeletal muscle using 10394-1-AP (same clone as 10394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using CFLAR/FLIP Polyclonal antibody (10394-1-AP)

    IHC staining of mouse skeletal muscle using 10394-1-AP (same clone as 10394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10394-1-AP (CFLAR/FLIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CFLAR/FLIP Polyclonal antibody (10394-1-AP)

    IF Staining of HeLa using 10394-1-AP (same clone as 10394-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CFLAR/FLIP antibody (10394-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 10394-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CFLAR/FLIP Polyclonal antibody, PBS Only

CFLAR/FLIP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10394-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10394-1-PBS targets CFLAR/FLIP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CFLAR/FLIP fusion protein Ag0593 相同性解析による交差性が予測される生物種
Full Name CASP8 and FADD-like apoptosis regulator
Calculated molecular weight 52 kDa
Observed molecular weight 20-35 kDa, 50-55 kDa
GenBank accession numberBC001602
Gene Symbol FLIP
Gene ID (NCBI) 8837
RRIDAB_2081087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15519
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CFLAR, also named as CASH, CASP8AP1, CLARP, MRIT, Casper, c-FLIP and I-FLICE, belongs to the peptidase C14A family. It is an apoptosis regulator protein which may function as a crucial link between cell survival and cell death pathways in mammalian cells. CFLAR acts as an inhibitor of TNFRSF6 mediated apoptosis. It can be cleaved to be 2 fragments P43 and P12. P43 is likely retained in the death-inducing signaling complex (DISC) thereby blocking further recruitment and processing of caspase-8 at the complex. Full length and shorter isoforms have been shown either to induce apoptosis or to reduce TNFRSF-triggered apoptosis. CFLAR lacks enzymatic (caspase) activity.