Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of human liver using 11848-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 11848-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human liver using 11848-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 11848-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 11848-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse skeletal muscle using 11848-1-AP
mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11848-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11848-1-AP (Cofilin 2 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human skeletal muscle using 11848-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human colon using 11848-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 11848-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11848-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 11848-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human heart using 11848-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 11848-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 11848-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11848-1-AP (Cofilin 2 antibody at dilution of 1:50 (under 40x lens).
IF Staining of L02 using 11848-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using Cofilin 2 antibody (11848-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Cofilin 2 Polyclonal antibody

Cofilin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11848-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CFL2, CFL 2, NEM7, Cofilin-2, Cofilin2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human liver tissue, L02 cells, mouse liver tissue, mouse lung tissue, mouse skeletal muscle tissue
Positive IHC detected in	human normal colon, human gliomas tissue, human heart tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	L02 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

11848-1-AP targets Cofilin 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2408 Product name: Recombinant human CFL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-166 aa of BC022876 Sequence: MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL 相同性解析による交差性が予測される生物種
Full Name	cofilin 2 (muscle)
Calculated molecular weight	166 aa, 19 kDa
Observed molecular weight	19 kDa
GenBank accession number	BC022876
Gene Symbol	Cofilin 2
Gene ID (NCBI)	1073
RRID	AB_2080914
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y281
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for Cofilin 2 antibody 11848-1-AP	Download protocol
IHC protocol for Cofilin 2 antibody 11848-1-AP	Download protocol
WB protocol for Cofilin 2 antibody 11848-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Mol Ther Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells. Authors - Lara Ottaviani View Article
human	WB,IHC,IF	Noncoding RNA Res ADAR1-regulated miR-142-3p/RIG-I axis suppresses antitumor immunity in nasopharyngeal carcinoma Authors - Haoyuan Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human liver using 11848-1-AP

WB analysis of mouse lung using 11848-1-AP

WB analysis of human liver using 11848-1-AP

WB analysis of L02 using 11848-1-AP

WB analysis of mouse skeletal muscle using 11848-1-AP

WB analysis of mouse skeletal muscle using 11848-1-AP

WB analysis of mouse liver using 11848-1-AP

IHC staining of human skeletal muscle using 11848-1-AP

IHC staining of human colon using 11848-1-AP

IHC staining of human colon using 11848-1-AP

IHC staining of human gliomas using 11848-1-AP

IHC staining of human heart using 11848-1-AP

IHC staining of human heart using 11848-1-AP

IHC staining of human heart using 11848-1-AP

IHC staining of human heart using 11848-1-AP

IF Staining of L02 using 11848-1-AP

Cofilin 2 Polyclonal antibody

Cofilin 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Intercellular transfer of miR-200c-3p impairs the angiogenic capacity of cardiac endothelial cells.

ADAR1-regulated miR-142-3p/RIG-I axis suppresses antitumor immunity in nasopharyngeal carcinoma