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  • KD/KO Validated

CES1 Polyclonal antibody

CES1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16912-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Liver carboxylesterase 1, CES 1, CEH, CE-1, Carboxylesterase 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:800-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16912-1-AP targets CES1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CES1 fusion protein Ag10567 相同性解析による交差性が予測される生物種
Full Name carboxylesterase 1 (monocyte/macrophage serine esterase 1)
Calculated molecular weight 566 aa, 62 kDa
Observed molecular weight 60-63 kDa
GenBank accession numberBC012418
Gene Symbol CES1
Gene ID (NCBI) 1066
RRIDAB_2077500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP23141
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CES1(liver carboxylesterase 1) is also named as SES1 and belongs to the type-B carboxylesterase/lipase family. The deduced 567-amino acid protein contains a putative 18-amino acid signal peptide and a characteristic C-terminal endoplasmic reticulum retention signal (HXEL). It is the major hydrolytic enzyme responsible for the metabolism of numerous therapeutic agents as well as endogenous substrates.Westernblot analysis demonstrated that CES1 is expressed in human liver microsomes (HLM) but not in human intestinal microsomes (HIM)(PMID:19185566).

Protocols

Product Specific Protocols
WB protocol for CES1 antibody 16912-1-APDownload protocol
IHC protocol for CES1 antibody 16912-1-APDownload protocol
IF protocol for CES1 antibody 16912-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Brain

ATAD3 gene cluster deletions cause cerebellar dysfunction associated with altered mitochondrial DNA and cholesterol metabolism.

Authors - Radha Desai
humanWB,IHC

Clin Transl Med

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Authors - Lijie Zhou
  • KO Validated
humanWB,IF

Oncotarget

Multifactorial resistance to aminopeptidase inhibitor prodrug CHR2863 in myeloid leukemia cells: down-regulation of carboxylesterase 1, drug sequestration in lipid droplets and pro-survival activation ERK/Akt/mTOR.

Authors - Sue Ellen Verbrugge
humanWB

Br J Pharmacol

PMID: 28128444

Authors - Wenjing Luo
humanWB

Drug Metab Dispos

Simultaneous absolute protein quantification of carboxylesterases 1 and 2 in human liver tissue fractions using liquid chromatography-tandem mass spectrometry.

Authors - Sato Yuichiro Y
mouseWB

J Pharm Pharmacol

Clock gene Bmal1 controls diurnal rhythms in expression and activity of intestinal carboxylesterase 1.

Authors - Xun Chen
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