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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse heart tissue using CERK Polyclonal antibody (25731-1-AP)

    WB analysis of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 25731-1-AP (CERK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using CERK Polyclonal antibody (25731-1-AP)

    WB analysis of mouse liver using 25731-1-AP (same clone as 25731-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25731-1-AP (CERK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CERK Polyclonal antibody (25731-1-AP)

    IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CERK Polyclonal antibody (25731-1-AP)

    IHC staining of mouse heart using 25731-1-AP (same clone as 25731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CERK Polyclonal antibody (25731-1-AP)

    IHC staining of human breast cancer using 25731-1-AP (same clone as 25731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CERK Polyclonal antibody (25731-1-AP)

    IHC staining of human breast cancer using 25731-1-AP (same clone as 25731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25731-1-AP (CERK antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25731-1-PBS in a different storage buffer formulation.

CERK Polyclonal antibody, PBS Only

CERK Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25731-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

25731-1-PBS targets CERK in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22774

Product name: Recombinant human CERK protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 224-537 aa of BC126940

Sequence: AVLVPSSLRIGIIPAGSTDCVCYSTVGTSDAETSALHIVVGDSLAMDVSSVHHNSTLLRYSVSLLGYGFYGDIIKDSEKKRWLGLARYDFSGLKTFLSHHCYEGTVSFLPAQHTVGSPRDRKPCRAGCFVCRQSKQQLEEEQKKALYGLEAAEDVEEWQVVCGKFLAINATNMSCACRRSPRGLSPAAHLGDGSSDLILIRKCSRFNFLRFLIRHTNQQDQFDFTFVEVYRVKKFQFTSKHMEDEDSDLKEGGKKRFGHICSSHPSCCCTVSNSSWNCDGEVLHSPAIEVRVHCQLVRLFARGIEENPKPDSHS

相同性解析による交差性が予測される生物種
Full Name ceramide kinase
Calculated molecular weight 537 aa, 60 kDa, 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC126940
Gene Symbol CERK
Gene ID (NCBI) 64781
RRIDAB_2880214
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TCT0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Ceramide kinase (CERK) is a crucial lipid kinase that specifically catalyses ceramide (CER) to ceramide-1-phosphate. It plays a vital role in biological functions and certain diseases, including aberrant wound healing (PMID: 24823941), obesity-diabetes (PMID: 22465662), mesangio-proliferativekidney diseases (PMID: 25134723), cancer (PMID: 25164007) and neuroblastoma (PMID: 22579669). CERK has 2 isoforms with the molecular weight of 60 and 38 kDa.

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