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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human liver tissue using CEPT1 Polyclonal antibody (20496-1-AP)

    IHC staining of human liver using 20496-1-AP (same clone as 20496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20496-1-AP (CEPT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20496-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CEPT1 Polyclonal antibody (20496-1-AP)

    IHC staining of human liver using 20496-1-AP (same clone as 20496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20496-1-AP (CEPT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20496-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CEPT1 Polyclonal antibody (20496-1-AP)

    IHC staining of human tonsillitis using 20496-1-AP (same clone as 20496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20496-1-AP (CEPT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20496-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CEPT1 Polyclonal antibody (20496-1-AP)

    IHC staining of human pancreas using 20496-1-AP (same clone as 20496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 20496-1-AP (CEPT1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20496-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CEPT1 Polyclonal antibody (20496-1-AP)

    IHC staining of human pancreas using 20496-1-AP (same clone as 20496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 20496-1-AP (CEPT1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20496-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CEPT1 Polyclonal antibody (20496-1-AP)

    IHC staining of human cervical cancer using 20496-1-AP (same clone as 20496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20496-1-AP (CEPT1 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20496-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CEPT1 Polyclonal antibody (20496-1-AP)

    IHC staining of human cervical cancer using 20496-1-AP (same clone as 20496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20496-1-AP (CEPT1 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20496-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CEPT1 Polyclonal antibody (20496-1-AP)

    IF Staining of HepG2 using 20496-1-AP (same clone as 20496-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using CEPT1 antibody 20496-1-AP at 1:25 dilution and FITC- labeled goat anti-Rabbit IgG (green). This data was developed using the same antibody clone with 20496-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CEPT1 Polyclonal antibody, PBS Only

CEPT1 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 20496-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

1-alkenyl-2-acylglycerol choline phosphotransferase, choline/ethanolamine phosphotransferase 1, EC:2.7.8.1, EC:2.7.8.22, hCEPT1

Formulation:  PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20496-1-PBS targets CEPT1 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14140

Product name: Recombinant human CEPT1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-91 aa of BC032610

Sequence: MSGHRSTRKRCGDSHPESPVGFGHMSTTGCVLNKLFQLPTPPLSRHQLKRLEEHRYQSAGRSLLEPLMQGYWEWLVRRVPSWIAPNLITII

相同性解析による交差性が予測される生物種
Full Name choline/ethanolamine phosphotransferase 1
Calculated molecular weight 416 aa, 47 kDa
GenBank accession numberBC032610
Gene Symbol CEPT1
Gene ID (NCBI) 10390
RRIDAB_10694283
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6K0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Choline-enthanolamine phosphotransferase 1 (CEPT1) is a 47-kDa membrane-bound protein that catalyzes the terminal step of the Kennedy pathway. CEPT1 is encoded by the Cept1 gene and is responsible for phosphatidylcholine (PC) and phosphatidylethanolamine (PE) production (PMID: 33214136, 30030520).

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