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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CEP83 Polyclonal antibody (26013-1-AP)

    WB analysis of HEK-293 using 26013-1-AP (same clone as 26013-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 26013-1-AP (CEP83 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CEP83 Polyclonal antibody (26013-1-AP)

    IHC staining of human kidney using 26013-1-AP (same clone as 26013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26013-1-AP (CCDC41 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using CEP83 Polyclonal antibody (26013-1-AP)

    IHC staining of human kidney using 26013-1-AP (same clone as 26013-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26013-1-AP (CCDC41 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CEP83 Polyclonal antibody (26013-1-AP)

    IF Staining of hTERT-RPE1 using 26013-1-AP (same clone as 26013-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CEP83 antibody (26013-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using CEP83 Polyclonal antibody (26013-1-AP)

    IF Staining of hTERT-RPE1 using 26013-1-AP (same clone as 26013-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP83 antibody (26013-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

CEP83 Polyclonal antibody, PBS Only

CEP83 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 26013-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

26013-1-PBS targets CEP83 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag22826

Product name: Recombinant human CCDC41 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 226-568 aa of BC125087

Sequence: MQATVRSLEAEKQSANLRAERLEKELQSSSEQNTFLINKLHKAEREINTLSSKVKELKHSNKLEITDIKLETARAKSELERERNKIQSELDGLQSDNEILKAAVEHHKVLLVEKDRELIRKVQAAKEEGYQKLVVLQDEKLELENRLADLEKMKVEHDVWRQSEKDQYEEKLRASQMAEEITRKELQSVRLKLQQQIVTIENAEKEKNENSDLKQQISSLQIQVTSLAQSENDLLNSNQMLKEMVERLKQECRNFRSQAEKAQLEAEKTLEEKQIQWLEEKHKLHERITDREEKYNQAKEKLQRAAIAQKKLEQDLELGCPSVTDTYRESVFPPPPLKRDLLK

相同性解析による交差性が予測される生物種
Full Name coiled-coil domain containing 41
Calculated molecular weight 693 aa, 82 kDa
Observed molecular weight 83 kDa, 68 kDa
GenBank accession numberBC125087
Gene Symbol CEP83
Gene ID (NCBI) 51134
RRIDAB_2880334
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y592
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CEP83 (centrosomal protein, 83 kDa), also known as CCDC41 (coiled-coil domain containing 41), is required for ciliary vesicle docking to the mother centriole. It is a key component of the centriolar distal appendages. CCDC41 may collaborate with IFT20 in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium during the initiation of primary cilium assembly (PMID: 23530209). Alternative splicing results in transcript variants encoding two isoforms with calculated molecular weights of 83 kDa and 68 kDa, respectively.

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