Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, human testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27031-1-AP targets CEP57 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24885 Product name: Recombinant human CEP57 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 241-500 aa of BC001233 Sequence: NRLIFEDKATPCVPNARRIKKKKSKPPEKKSSRNYFGAQPHYRLCLGDMPFVAGKSTSPSHAVVANVQLVLHLMKQHSKALCNDRVINSIPLAKQVSSRGGKSKKLSVTPPSSNGINEELSEVLQTLQDEFGQMSFDHQQLAKLIQESPTVELKDKLECELEALVGRMEAKANQITKVRKYQAQLEKQKLEKQKKELKATKKTLDEERNSSSRSGITGTTNKKDFMKLRPGEKRRKNLQLLKDMQSIQNSLQSSSLCWDY 相同性解析による交差性が予測される生物種 |
| Full Name | centrosomal protein 57kDa |
| Observed molecular weight | 57 kDa |
| GenBank accession number | BC001233 |
| Gene Symbol | CEP57 |
| Gene ID (NCBI) | 9702 |
| RRID | AB_3742211 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86XR8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cep57 is an ubiquitously-expressed protein that functions in spindle assembly, spindle pole integrity and central spindle organization as a microtubule- and centrosome-associated protein. Also, Cep57 is required for centriole duplication, and is highly expressed in prostate cancer cells.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CEP57 antibody 27031-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |