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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEP192 Polyclonal antibody (28700-1-AP)

    WB analysis using 28700-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28700-1-AP (CEP192 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP192 Polyclonal antibody (28700-1-AP)

    IF Staining of HeLa using 28700-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 28700-1-AP (CEP192 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP192 Polyclonal antibody (28700-1-AP)

    IF Staining of HeLa using 28700-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP192 antibody (28700-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CEP192 Polyclonal antibody

CEP192 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28700-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28700-1-AP targets CEP192 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag29930

Product name: Recombinant human CEP192 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 768-988 aa of NM_032142

Sequence: NDLEKSNGSNALDMEKYLKKTEVSRYESALENFSRASMSDTWDLSLPKEQTTQDIHPVDLSATSVSVRAPEENTAAIVYVENGESENQESFRTINSSNSVTNRENNSAVVDVKTCSIDNKLQDVGNDEKATSISTPSDSYSSVRNPRITSLCLLKDCEEIRDNRENQRQNECVSEISNSEKHVTFENHRIVSPKNSDLKNTSPEHGGRGSEDEQESFRPST

相同性解析による交差性が予測される生物種
Full Name centrosomal protein 192kDa
Calculated molecular weight 297 kDa
Observed molecular weight280-300 kDa
GenBank accession numberNM_032142
Gene Symbol CEP192
Gene ID (NCBI) 55125
RRIDAB_2918191
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEP8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEP192, also named PP8407 and KIAA1569, interacts with SHBG. It is required for mitotic centrosome and spindle assembly. CEP192 appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication.

Protocols

Product Specific Protocols
IF protocol for CEP192 antibody 28700-1-APDownload protocol
WB protocol for CEP192 antibody 28700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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