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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEP152 Polyclonal antibody (21815-1-AP)

    WB analysis using 21815-1-AP (same clone as 21815-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21815-1-AP (CEP152 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CEP152 Polyclonal antibody (21815-1-AP)

    WB analysis of mouse brain using 21815-1-AP (same clone as 21815-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21815-1-AP (CEP152 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using CEP152 Polyclonal antibody (21815-1-AP)

    IP experiment of mouse brain using 21815-1-AP (same clone as 21815-1-PBS)

    IP result of anti-CEP152 (IP:21815-1-AP, 4ug; Detection:21815-1-AP 1:2000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CEP152 Polyclonal antibody (21815-1-AP)

    IHC staining of human brain using 21815-1-AP (same clone as 21815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21815-1-AP (CEP152 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using CEP152 Polyclonal antibody (21815-1-AP)

    IHC staining of human brain using 21815-1-AP (same clone as 21815-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21815-1-AP (CEP152 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CEP152 Polyclonal antibody, PBS Only

CEP152 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 21815-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

KIAA0912, centrosomal protein 152kDa, CEP 152

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21815-1-PBS targets CEP152 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name centrosomal protein 152kDa
Calculated molecular weight 189 kDa
Observed molecular weight 66 kDa
GenBank accession numberNM_014985
Gene Symbol CEP152
Gene ID (NCBI) 22995
RRIDAB_2878921
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO94986
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CEP152, also named as KIAA0912, is a regulator of genomic integrity and cellular response to DNA damage acting through ATR-mediated checkpoint signaling. It is necessary for centrosome duplication. CEP152 functions as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, two molecules involved in centriole formation. CEP152, ATR and CENPJ control cellular responses to DNA damage. CEP152 has some isoforms with MW 189 kDa, 152 kDa, 110 kDa and 66 kDa.

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