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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293T cells using CEP128 Polyclonal antibody (24810-1-AP)

    WB analysis of HEK-293T using 24810-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 24810-1-AP (CEP128 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using CEP128 Polyclonal antibody (24810-1-AP)

    IF Staining of U2OS using 24810-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using CEP128 antibody (24810-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP128 Polyclonal antibody (24810-1-AP)

    IF Staining of HeLa using 24810-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP128 antibody (24810-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CEP63 antibody (66996-1-Ig, Clone: 2G12B2, red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEP128 Polyclonal antibody (24810-1-AP)

    IF Staining of HeLa using 24810-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP128 antibody (24810-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CEP63 antibody (66996-1-Ig, Clone: 2G12B2, red).

CEP128 Polyclonal antibody

CEP128 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24810-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C14orf145, Centrosomal protein of 128 kDa, C14orf61

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells
Positive IF/ICC detected inHeLa cells, U2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24810-1-AP targets CEP128 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20610

Product name: Recombinant human C14orf145 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-435 aa of BC045834

Sequence: MAESSSESDHFRCRDRLSPWAARSTHRGTRSLPTVEVTEKVNTITSTLQDTSRNLRQVDQMLGRYREYSNGQAGAIEHLKESLEQSIDQLRSQRLLRNSGGRSISVTSLSASDLDGGTGSELHHFPPTSPLKDYGDPQGIKRMRSRTGVRFVQETDDMTQLHGFHQSLRDLSSEQIRLGDDFNRELSRRSRSDAETKRALEELTEKLNEAQKQEVVSDRVERRLQELEREMRTERELVERRQDQLGLMSLQLQEALKKQEAKADEHEGAIKNKLRQTETEKNQLEQELELSRRLLNQSEGSRETLLHQVEELRTQLTKAEGDRKGLQHQVSQISKQQSNYQDEQGEDWRFRRGVEREKQDLEKQMSDLRVQLNFSAMASELEEVKRCMERKDKERAHLASQVEHINRKCLLNLVQDLDCKDNEILTYSLQSPLHV

相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 145
Calculated molecular weight 1094 aa, 128 kDa
Observed molecular weight128 kDa
GenBank accession numberBC045834
Gene Symbol CEP128
Gene ID (NCBI) 145508
RRIDAB_2923573
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6ZU80
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Centrosomal protein of 128kDa is encoded by CEP128 gene in humans. CEP128 localizes to the subdistal appendages of the mother centriole and regulates TGF-β/BMP signaling at the primary cilium (PMID: 29514088). Centrosomal protein CEP128 plays a role in male fertility and its variants affected the ciliogenesis in testes (PMID: 35296684).

Protocols

Product Specific Protocols
IF protocol for CEP128 antibody 24810-1-APDownload protocol
WB protocol for CEP128 antibody 24810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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