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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using CENPO Polyclonal antibody (20611-1-AP)

    WB analysis of mouse brain using 20611-1-AP (same clone as 20611-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CENPO Polyclonal antibody (20611-1-AP)

    WB analysis of HEK-293 using 20611-1-AP (same clone as 20611-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse ovary tissue using CENPO Polyclonal antibody (20611-1-AP)

    WB analysis of mouse ovary using 20611-1-AP (same clone as 20611-1-PBS)

    mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse kidney tissue using CENPO Polyclonal antibody (20611-1-AP)

    WB analysis of mouse kidney using 20611-1-AP (same clone as 20611-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CENPO Polyclonal antibody (20611-1-AP)

    WB analysis of mouse brain using 20611-1-AP (same clone as 20611-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CENPO Polyclonal antibody (20611-1-AP)

    WB analysis using 20611-1-AP (same clone as 20611-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

CENPO Polyclonal antibody, PBS Only

CENPO Polyclonal Antibody for WB, Indirect ELISA
Cat No. 20611-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20611-1-PBS targets CENPO in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14619

Product name: Recombinant human CENPO protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC002870

Sequence: MEQANPLRPDGESKGGVLAHLERLETQVSRSRKQSEELQSVQAQEGALGTKIHKLRRLRDELRAVVRHRRASVKACIANVEPNQTVEINEQEALEEKLENVKAILQAYHFTGLSGKLTSRGVCVCISTAFEGNLLDSYFVDLVIQKPLRIHHHSVPVFIPLEEIAAKYLQTNIQHFLFSLCEYLNAYSGRKYQADRLQSDFAALLTGPLQRNPLCNLLSFTYKLDPGGQSFPFCARLLYKDLTATLPTDVTVTCQGVEVLSTSWEEQRASHETLFCTKPLHQVFASFTRKGEKLDMSLVS

相同性解析による交差性が予測される生物種
Full Name centromere protein O
Calculated molecular weight 300 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC002870
Gene Symbol CENPO
Gene ID (NCBI) 79172
RRIDAB_10696179
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BU64
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CENPO is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. CENPO may be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Besides, CENPA modulates the kinetochore-bound levels of NDC80 complex. CENPO has 2 isoforms with MW of 33 and 34 kDa, while different lysates may bind to one or two isforms in SDS-PAGE.

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