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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CENPM Polyclonal antibody (19840-1-AP)

    WB analysis using 19840-1-AP (same clone as 19840-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19840-1-AP (CENPM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19840-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CENPM Polyclonal antibody (19840-1-AP)

    IF Staining of HeLa using 19840-1-AP (same clone as 19840-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CENPM antibody (19840-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19840-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CENPM Polyclonal antibody (19840-1-AP)

    IF Staining of NIH/3T3 using 19840-1-AP (same clone as 19840-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CENPM antibody (19840-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 19840-1-PBS in a different storage buffer formulation.

CENPM Polyclonal antibody, PBS Only

CENPM Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 19840-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19840-1-PBS targets CENPM in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13843

Product name: Recombinant human CENPM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-180 aa of BC000705

Sequence: MSVLRPLDKLPGLNTATILLVGTEDALLQQLADSMLKEDCASELKVHLAKSLPLPSSVNRPRIDLIVFVVNLHSKYSLQNTEESLRHVDASFFLGKVCFLATGAGRESHCSIHRHTVVKLAHTYQSPLLYCDLEVEGFRATMAQRLVRVLQICAGHVPGVSALNLLSLLRSSEGPSLEDL

相同性解析による交差性が予測される生物種
Full Name centromere protein M
Calculated molecular weight 180 aa, 20 kDa
Observed molecular weight20 kDa
GenBank accession numberBC000705
Gene Symbol CENPM
Gene ID (NCBI) 79019
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSP4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Centromere protein M (CENPM), also referred as proliferation associated nuclear element 1 (PANE1), is one of the components of a complex that plays a central role in kinetochore protein assembly, mitotic progression and chromosome segregation. The expression of CENPM and centromere assembly should be controlled closely during the cell cycle, and mistakes in this regulation may result in aneuploidy (PMID: 32705259). CENPM has been reported as a novel biomarker of hepatocellular carcinoma, melanoma and bladder cancer (PMID: 31703591).

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