Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, mouse brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86805-3-RR targets CENPE in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag27230 Product name: Recombinant human CENPE protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2401-2500 aa of NM_001286734 Sequence: MESKIIKMQKELEVTNDIIAKLQAKVHESNKCLEKTKETIQVLQDKVALGAKPYKEEIEDLKMKLVKIDLEKMKNAKEFEKEISATKATVEYQKEVIRLLR 相同性解析による交差性が予測される生物種 |
| Full Name | centromere protein E, 312kDa |
| Calculated molecular weight | 316 kDa |
| Observed molecular weight | 300-316 kDa |
| GenBank accession number | NM_001286734 |
| Gene Symbol | CENPE |
| Gene ID (NCBI) | 1062 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q02224 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Centromeric protein E (CENPE) is also named Kinesin-7. CENPE, a member of the centromere protein (CENP) family, functions as a microtubule plus-end directed motor protein that is upregulated during the G2 phase (PMID: 31115500, PMID: 7851898, PMID: 38560520). It plays a crucial role in the spindle assembly checkpoint (SAC), chromosome alignment, and microtubule capture at kinetochores during mitosis (PMID: 30784092). CENPE is essential for mitotic white matter, which accumulates during the G2 phase of the cell cycle, is consumed throughout mitosis and degrades later in the cell cycle (PMID: 33862458). CENPE regulates the cell cycle by targeting the transcription of CDK1 in the nucleus (PMID: 33862458). Studies on tumor cells have shown that CENPE plays an important role in the regulation of cell proliferation, apoptosis, migration and other activities (PMID: 33862458).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CENPE antibody 86805-3-RR | Download protocol |
| WB protocol for CENPE antibody 86805-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |