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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CENPB Polyclonal antibody (26738-1-AP)

    WB analysis using 26738-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26738-1-AP (CENPB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CENPB Polyclonal antibody

CENPB Polyclonal Antibody for WB, ELISA
Cat No. 26738-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CENP B, CENPB, Centromere protein B, centromere protein B, 80kDa, Major centromere autoantigen B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26738-1-AP targets CENPB in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24967

Product name: Recombinant human CENPB protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-180 aa of BC053847

Sequence: MGPKRRQLTFREKSRIIQEVEENPDLRKGEIARRFNIPPSTLSTILKNKRAILASERKYGVASTCRKTNKLSPYDKLEGLLIAWFQQIRAAGLPVKGIILKEKALRIAEELGMDDFTASNGWLDRFRRRHGVVSCSGVARARARNAAPRTPAAPASPAAVPSEGSGGSTTGWRAREEQPP

相同性解析による交差性が予測される生物種
Full Name centromere protein B, 80kDa
Calculated molecular weight 65 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC053847
Gene Symbol CENPB
Gene ID (NCBI) 1059
RRIDAB_3085899
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP07199
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Centromere protein B (CENPB), also known as major centromere autoantigen B, is an autoantigen protein of the cell nucleus. In mammalian centromeres CENPB plays a critical role in the organization of chromatinstructure, but in the absence of CENPB some chromosomes use other redundant proteins to accomplish mitosis (PMID: 14963831). CENPB plays an important role in cell cycle regulation. CENPB is highly expressed in cancer cells helping in high rate proliferation. This antibody detects CENPB protein with a molecular weight of 80 kDa (PMID: 34547289, PMID: 29273057).

Protocols

Product Specific Protocols
WB protocol for CENPB antibody 26738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols