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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using CELSR2 Recombinant antibody (83115-1-RR)

    IF Staining of SH-SY5Y using 83115-1-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CELSR2 antibody (83115-1-RR, Clone: 230319H5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HeLa cells using CELSR2 Recombinant monoclonal antibody (83115-1-RR)

    FC experiment of HeLa using 83115-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CELSR2 Recombinant antibody (83115-1-RR, Clone:230319H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CELSR2 Recombinant monoclonal antibody

CELSR2 Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83115-1-RR
Clone No.230319H5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

230319H5, Cadherin EGF LAG seven-pass G-type receptor 2, Cadherin family member 10, CDHF10, EGF like protein 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83115-1-RR targets CELSR2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cadherin, EGF LAG seven-pass G-type receptor 2 (flamingo homolog, Drosophila)
Calculated molecular weight 318 kDa
GenBank accession numberNM_001408
Gene Symbol CELSR2
Gene ID (NCBI) 1952
RRIDAB_3670827
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9HCU4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CELSR2, also named CDHF10, EGFL2, KIAA0279, and MEGF3, belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is a receptor that may be important in cell/cell signaling during nervous system formation.

Protocols

Product Specific Protocols
IF protocol for CELSR2 antibody 83115-1-RRDownload protocol
FC protocol for CELSR2 antibody 83115-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols