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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using CEBPE Recombinant antibody (86432-1-RR)

    WB analysis of HL-60 using 86432-1-RR

    HL-60 cells were subjected to SDS PAGE followed by western blot with 86432-1-RR (CEBPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CEBPE Recombinant antibody (86432-1-RR)

    WB analysis of Jurkat using 86432-1-RR

    Jurkat cells were subjected to SDS PAGE followed by western blot with 86432-1-RR (CEBPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CEBPE Recombinant antibody (86432-1-RR)

    IF Staining of HepG2 using 86432-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEBPE antibody (86432-1-RR, Clone: 251303B10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CEBPE Recombinant monoclonal antibody (86432-1-RR)

    IF Staining of HeLa using 86432-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CEBPE antibody (86432-1-RR, Clone: 251303B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CEBPE Recombinant monoclonal antibody (86432-1-RR)

    IF Staining of A431 using 86432-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CEBPE antibody (86432-1-RR, Clone: 251303B10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CEBPE Recombinant monoclonal antibody

CEBPE Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86432-1-RR
Clone No.251303B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

C/EBP epsilon, CCAAT/enhancer-binding protein epsilon, CRP1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, HL-60 cells
Positive IF/ICC detected inHepG2 cells, A431 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86432-1-RR targets CEBPE in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag5589

Product name: Recombinant human CEBPE protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-281 aa of BC035797

Sequence: MSHGTYYECEPRGGQQPLEFSGGRAGPGELGDMCEHEASIDLSAYIESGEEQLLSDLFAVKPAPEARGLKGPGTPAFPHYLPPDPRPFAYPPHTFGPDRKALGPGIYSSPGSYDPRAVAVKEEPRGPEGSRAASRGSYNPLQYQVAHCGQTAMHLPPTLAAPGQPLRVLKAPLATAAPPCSPLLKAPSPAGPLHKGKKAVNKDSLEYRLRRERNNIAVRKSRDKAKRRILETQQKVLEYMAENERLRSRVEQLTQELDTLRNLFRQIPEAANLIKGVGGCS

相同性解析による交差性が予測される生物種
Full Name CCAAT/enhancer binding protein (C/EBP), epsilon
Calculated molecular weight 31 kDa
Observed molecular weight 31-38 kDa
GenBank accession numberBC035797
Gene Symbol CEBPE
Gene ID (NCBI) 1053
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ15744
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEBPE (CCAAT/enhancer binding protein epsilon), also known as C/EBP-epsilon and CRP1, is a transcription factor involved in late myeloid lineage differentiation and cellular function (PMID: 31201888). CEBPE is expressed in a stage-specific manner during myeloid differentiation and regulates transition from the promyelocyte to the myelocyte (PMID: 29773596). Depletion of CEBPE in Acute lymphoblastic leukaemia (ALL) cells reduces cell growth, correspondingly CEBPE binds to the promoters of electron transport and energy generation genes (PMID: 29977016).

Protocols

Product Specific Protocols
IF protocol for CEBPE antibody 86432-1-RRDownload protocol
WB protocol for CEBPE antibody 86432-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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