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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CEACAM-1/CD66a Polyclonal antibody (32395-1-AP)

    WB analysis using 32395-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32395-1-AP (CEACAM-1/CD66a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CEACAM-1/CD66a Polyclonal antibody (32395-1-AP)

    IHC staining of mouse spleen using 32395-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32395-1-AP (CEACAM-1/CD66a antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using CEACAM-1/CD66a Polyclonal antibody (32395-1-AP)

    IHC staining of mouse spleen using 32395-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32395-1-AP (CEACAM-1/CD66a antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using CEACAM-1/CD66a Polyclonal antibody (32395-1-AP)

    IF Staining of mouse spleen using 32395-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CEACAM-1/CD66a antibody (32395-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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CEACAM-1/CD66a Polyclonal antibody

CEACAM-1/CD66a Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 32395-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF-P, ELISA

Synonyms

Carcinoembryonic antigen-related cell adhesion molecule 1, Biliary glycoprotein D, Biliary glycoprotein 1, BGP-1, Bgp1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, mouse liver tissue, mouse small intestine
Positive IF-P detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32395-1-AP targets CEACAM-1/CD66a in WB, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name carcinoembryonic antigen-related cell adhesion molecule 1
Calculated molecular weight57 kDa
Observed molecular weight100-120 kDa
Gene Symbol Ceacam1
Gene ID (NCBI) 26365
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP31809-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CEACAM1, also known as CD66a or BGP (biliary glycoprotein), is a member of the cacinoembryonic antigen (CEA) family which belongs to the immunoglobulin superfamily of cell adhesion molecules (PMID: 16919437). CEACAMs play major roles in cell-cell and cell-ECM adhesion and have been implicated in the control of cell proliferation, angiogenesis, and tissue remodeling (PMID: 23021083). CEACAM1 is a transmembrane glycoprotein that is expressed on epithelial, endothelial, and immune cells (PMID: 30314283). CEACAM1 has been demonstrated to play a role in morphogenesis, apoptosis, angiogenesis, cell proliferation, cell motility, fibrosis, and immunity (PMID: 31604530).

Protocols

Product Specific Protocols
WB protocol for CEACAM-1/CD66a antibody 32395-1-APDownload protocol
IF protocol for CEACAM-1/CD66a antibody 32395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols