Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of PC-3 using 17763-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 17763-1-AP (CDYL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 17763-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 17763-1-AP (CDYL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 17763-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 17763-1-AP (CDYL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 17763-1-AP
IP result of anti-CDYL (IP:17763-1-AP, 4ug; Detection:17763-1-AP 1:1000) with MCF-7 cells lysate 3600 ug.
IHC staining of human breast cancer using 17763-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 17763-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 17763-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 17763-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17763-1-AP (CDYL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

CDYL Polyclonal antibody

CDYL Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 17763-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

CDY like, CDYL, CDYL1, chromodomain protein, Y like, Chromodomain Y like protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	PC-3 cells, mouse testis tissue, MCF-7 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human breast cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 6 publications below
IHC	See 2 publications below
IF	See 2 publications below

Product Information

17763-1-AP targets CDYL in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12143 Product name: Recombinant human CDYL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-362 aa of BC108725 Sequence: MASEELYEVERIVDKRKNKKGKTEYLVRWKGYDSEDDTWEPEQHLVNCEEYIHDFNRRHTEKQKESTLTRTNRTSPNNARKQISRSTNSNFSKTSPKALVIGKDHESKNSQLFAASQKFRKNTAPSLSSRKNMDLAKSGIKILVPKSPVKSRTAVDGFQSESPEKLDPVEQGQEDTVAPEVAAEKPVGALLGPGAERARMGSRPRIHPLVPQVPGPVTAAMATGLAVNGKGTSPFMDALTANGTTNIQTSVTGVTASKRKFIDDRRDQPFDKRLRFSVRQTESAYRYRDIVVRKQDGFTHILLSTKSSENNSLNPEVMREVQSALSTAAADDSKLVLLSAVGSVFCCGLDFIYFIRRLTDDR 相同性解析による交差性が予測される生物種
Full Name	chromodomain protein, Y-like
Calculated molecular weight	544 aa, 61 kDa
Observed molecular weight	66 kDa, 60 kDa
GenBank accession number	BC108725
Gene Symbol	CDYL
Gene ID (NCBI)	9425
RRID	AB_2077197
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y232
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for CDYL antibody 17763-1-AP	Download protocol
IP protocol for CDYL antibody 17763-1-AP	Download protocol
WB protocol for CDYL antibody 17763-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Endocrinology Dynamic profiles and transcriptional preferences of histone modifications during spermiogenesis. Authors - Yinchuan Li View Article
mouse	WB	Adv Sci (Weinh) Cdyl Deficiency Brakes Neuronal Excitability and Nociception through Promoting Kcnb1 Transcription in Peripheral Sensory Neurons. Authors - Zhao-Wei Sun View Article
human	WB	Transl Psychiatry Activity-dependent phosphorylation of CDYL by CDK5 regulates fear memory in mice Authors - Na-Yun Lyu View Article
human	WB	Cancer Lett CDYL knockdown reduces glioma development through an antitumor immune response in the tumor microenvironment Authors - Jin-Ge Kong KD Validated View Article
		Andrology Aberrant acetylation caused by a CDYL splicing mutation contributes to thin mid-piece related asthenoteratozoospermia and male infertility Authors - Fan Yang View Article
human,mouse	WB,IHC,IF	Acta Pharmacol Sin Chromodomain Y-like (CDYL) inhibition ameliorates acute kidney injury in mice by regulating tubular pyroptosis Authors - Ting Xiang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of PC-3 using 17763-1-AP

WB analysis of mouse testis using 17763-1-AP

WB analysis of MCF-7 using 17763-1-AP

IP experiment of MCF-7 using 17763-1-AP

IHC staining of human breast cancer using 17763-1-AP

IHC staining of human breast cancer using 17763-1-AP

IHC staining of human lung cancer using 17763-1-AP

IHC staining of human lung cancer using 17763-1-AP

CDYL Polyclonal antibody

CDYL Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Dynamic profiles and transcriptional preferences of histone modifications during spermiogenesis.

Cdyl Deficiency Brakes Neuronal Excitability and Nociception through Promoting Kcnb1 Transcription in Peripheral Sensory Neurons.

Activity-dependent phosphorylation of CDYL by CDK5 regulates fear memory in mice

CDYL knockdown reduces glioma development through an antitumor immune response in the tumor microenvironment

Aberrant acetylation caused by a CDYL splicing mutation contributes to thin mid-piece related asthenoteratozoospermia and male infertility

Chromodomain Y-like (CDYL) inhibition ameliorates acute kidney injury in mice by regulating tubular pyroptosis

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