Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, mouse testis tissue, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
17763-1-AP targets CDYL in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12143 Product name: Recombinant human CDYL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-362 aa of BC108725 Sequence: MASEELYEVERIVDKRKNKKGKTEYLVRWKGYDSEDDTWEPEQHLVNCEEYIHDFNRRHTEKQKESTLTRTNRTSPNNARKQISRSTNSNFSKTSPKALVIGKDHESKNSQLFAASQKFRKNTAPSLSSRKNMDLAKSGIKILVPKSPVKSRTAVDGFQSESPEKLDPVEQGQEDTVAPEVAAEKPVGALLGPGAERARMGSRPRIHPLVPQVPGPVTAAMATGLAVNGKGTSPFMDALTANGTTNIQTSVTGVTASKRKFIDDRRDQPFDKRLRFSVRQTESAYRYRDIVVRKQDGFTHILLSTKSSENNSLNPEVMREVQSALSTAAADDSKLVLLSAVGSVFCCGLDFIYFIRRLTDDR 相同性解析による交差性が予測される生物種 |
| Full Name | chromodomain protein, Y-like |
| Calculated molecular weight | 544 aa, 61 kDa |
| Observed molecular weight | 66 kDa, 60 kDa |
| GenBank accession number | BC108725 |
| Gene Symbol | CDYL |
| Gene ID (NCBI) | 9425 |
| RRID | AB_2077197 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y232 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDYL antibody 17763-1-AP | Download protocol |
| IHC protocol for CDYL antibody 17763-1-AP | Download protocol |
| IP protocol for CDYL antibody 17763-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Endocrinology Dynamic profiles and transcriptional preferences of histone modifications during spermiogenesis. | ||
Adv Sci (Weinh) Cdyl Deficiency Brakes Neuronal Excitability and Nociception through Promoting Kcnb1 Transcription in Peripheral Sensory Neurons. | ||
Drug Des Devel Ther Sorafenib Attenuates Fibrotic Hepatic Injury Through Mediating Lysine Crotonylation | ||
Cancer Lett CDYL knockdown reduces glioma development through an antitumor immune response in the tumor microenvironment
| ||
Acta Pharmacol Sin Chromodomain Y-like (CDYL) inhibition ameliorates acute kidney injury in mice by regulating tubular pyroptosis | ||
Cell Rep The chromodomain protein CDYL confers forebrain identity to human cortical organoids by inhibiting neuronatin
|