Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33231-1-AP targets CDY1B in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag37962 Product name: Recombinant human CDY1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 65-300 aa of BC132929 Sequence: LTWTTTSRIFSNNARRRTSRSTKANYSKNSPKTPVTDKHHRSKNRKLFAASKNVRRKAASILSDTKNMEIINSTIETLAPDSPFDHKTVSGFQKLEKLDPIAADQQDTVVFKVTEGKLLRDPLSRPGAEQTGIQNKTQIHPLMSQMSGSVTASMATGSATRKGIVVLIDPLAANGTTDMHTSVPRVKGGQRNITDDSRDQPFIKKMHFTIRLTESASTYRDIVVKKEDGFTQIVLS 相同性解析による交差性が予測される生物種 |
| Full Name | chromodomain protein, Y-linked, 1B |
| Calculated molecular weight | 540 aa, 60 kDa/554 aa, 62 kDa |
| Observed molecular weight | 65 kDa |
| GenBank accession number | BC132929 |
| Gene Symbol | CDY1B |
| Gene ID (NCBI) | 253175 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q9Y6F8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The CDY1B gene, also known as Chromodomain Y-Linked 1B, is a Protein Coding gene located on the Y chromosome. It is one of the two identical copies of the CDY1 gene within the AZFc region of the human Y chromosome. As a histone acetyltransferase, CDY1B exhibits a strong preference for histone 4, suggesting its involvement in both spermatogenic histone replacement and DNA transcription. It plays a significant role in the chromatin remodeling of round spermatid nuclei, contributing to the transition from histone-based chromatin to protamine-based chromatin during spermatogenesis. This transition is essential for the condensation of sperm DNA and the formation of mature sperm. Deletion of either CDY1 can lead to spermatogenic failure and male infertility.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDY1B antibody 33231-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |