Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse skin tissue, rat skin tissue |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85384-2-RR targets CDSN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg10164 Product name: Recombinant Human CDSN protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 33-529 aa of NM_001264.5 Sequence: KSIGTFSDPCKDPTRITSPNDPCLTGKGDSSGFSSYSGSSSSGSSISSARSSGGGSSGSSSGSSIAQGGSAGSFKPGTGYSQVSYSSGSGSSLQGASGSSQLGSSSSHSGNSGSHSGSSSSHSSSSSSFQFSSSSFQVGNGSALPTNDNSYRGILNPSQPGQSSSSSQTFGVSSSGQSVSSNQRPCSSDIPDSPCSGGPIVSHSGPYIPSSHSVSGGQRPVVVVVDQHGSGAPGVVQGPPCSNGGLPGKPCPPITSVDKSYGGYEVVGGSSDSYLVPGMTYSKGKIYPVGYFTKENPVKGSPGVPSFAAGPPISEGKYFSSNPIIPSQSAASSAIAFQPVGTGGVQLCGGGSTGSKGPCSPSSSRVPSSSSISSSSGLPYHPCGSASQSPCSPPGTGSFSSSSSSQSSGKIILQPCGSKSSSSGHPCMSVSSLTLTGGPDGSPHPDPSAGAKPCGSSSAGKIPCRSIRDILAQVKPLGPQLADPEVFLPQGELLNSP 相同性解析による交差性が予測される生物種 |
| Full Name | corneodesmosin |
| Calculated molecular weight | 52 kDa |
| Observed molecular weight | 55-65 kDa |
| GenBank accession number | NM_001264.5 |
| Gene Symbol | CDSN |
| Gene ID (NCBI) | 1041 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15517 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDSN, also named as S protein, is important for the epidermal barrier integrity. CDSN is a unique extracellular 52- to 56-kDa glycoprotein found in corneodesmosomes. Pathogenic mutations in CDSN have been linked in humans to genodermatoses affecting both the hair follicle and the epidermis. Inflammatory peeling skin syndrome is caused a novel mutation in CDSN.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CDSN antibody 85384-2-RR | Download protocol |
| WB protocol for CDSN antibody 85384-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |