Validation Data Gallery
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Tested Applications
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
Product Information
12877-1-AP targets p15 INK4b in WB, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3557 Product name: Recombinant human CDKN2B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-138 aa of BC014469 Sequence: MREENKGMPSGGGSDEGLASAAARGLVEKVRQLLEAGADPNGVNRFGRRAIQVMMMGSARVAELLLLHGAEPNCADPATLTRPVHDAAREGFLDTLVVLHRAGARLDVRDAWGRLPVDLAEERGHRDVAGYLRTATGD 相同性解析による交差性が予測される生物種 |
| Full Name | cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4) |
| Calculated molecular weight | 138 aa, 15 kDa |
| GenBank accession number | BC014469 |
| Gene Symbol | p15 INK4b/CDKN2B |
| Gene ID (NCBI) | 1030 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42772 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for p15 INK4b antibody 12877-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene CDKN2B deletion is essential for pancreatic cancer development instead of unmeaningful co-deletion due to juxtaposition to CDKN2A. | ||
EMBO Rep MYC promotes cancer progression by modulating m6 A modifications to suppress target gene translation. | ||
Cell Cycle Cell proliferation downregulated by TGF-β2-triggered G1/S checkpoint in clinical CAFs. | ||
Pancreatology Smad4 deficiency substitutes Cdkn2b but not Cdkn2a downregulation in pancreatic cancer following induction of genetic events in adult mice. | ||
PLoS One NRF2-regulated cell cycle arrest at early stage of oxidative stress response mechanism. | ||
Dis Model Mech A novel pancreatic cancer model originated from transformation of acinar cells in adult tree shrew, a primate-like animal. |
