Validation Data Gallery

Filter: AllWBIPIHCFC (Intra)

WB analysis using 22067-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 22067-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22067-1-AP (CDK8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 22067-1-AP
WB result of CDK8 antibody (22067-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK8 transfected HEK-293 cells.
IP experiment of HEK-293 using 22067-1-AP
IP result of anti-CDK8 (IP:22067-1-AP, 4ug; Detection:22067-1-AP 1:2000) with HEK-293 cells lysate 1320 ug.
IP experiment of NIH/3T3 using 22067-1-AP
IP result of anti-CDK8 (IP:22067-1-AP, 4ug; Detection:22067-1-AP 1:2000) with NIH/3T3 cells lysate 1160 ug.
IHC staining of human stomach cancer using 22067-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human stomach cancer using 22067-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human testis using 22067-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 22067-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 22067-1-AP (CDK8 antibody) at dilution of 1:50 (under 40x lens).
FC experiment of NIH/3T3 using 22067-1-AP
1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug CDK8 Polyclonal antibody (22067-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Featured Product
KD/KO Validated

CDK8 Polyclonal antibody

CDK8 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Cat No. 22067-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Synonyms

Cell division protein kinase 8, Cyclin dependent kinase 8, Cyclin-dependent kinase 8, EC:2.7.11.22, K35

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, NIH/3T3 cells, mouse embryo tissue
Positive IP detected in	NIH/3T3 cells, HEK-293 cells
Positive IHC detected in	human stomach cancer tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC (Intra) detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 6 publications below
IHC	See 2 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

22067-1-AP targets CDK8 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17148 Product name: Recombinant human CDK8 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 360-464 aa of BC069634 Sequence: TEEEPDDKGDKNQQQQQGNNHTNGTGHPGNQDSSHTQGPPLKKVRVVPPTTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSSMGYSATSQQPPQYSHQTHRY 相同性解析による交差性が予測される生物種
Full Name	cyclin-dependent kinase 8
Calculated molecular weight	464 aa, 53 kDa
Observed molecular weight	53 kDa
GenBank accession number	BC069634
Gene Symbol	CDK8
Gene ID (NCBI)	1024
RRID	AB_2878983
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49336
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
FC protocol for CDK8 antibody 22067-1-AP	Download protocol
IHC protocol for CDK8 antibody 22067-1-AP	Download protocol
IP protocol for CDK8 antibody 22067-1-AP	Download protocol
WB protocol for CDK8 antibody 22067-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB,IP,IF	J Adv Res CDK8 mediated inflammatory microenvironment aggravates osteoarthritis progression Authors - Zhongnan Lin KD Validated View Article
human	WB	Nucleic Acids Res Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells. Authors - Lindsey M Jackson KD Validated View Article
		EMBO J CDK8 remodels the tumor microenvironment and promotes resistance to KRAS(G12D) inhibitors and daraxonrasib in PDAC. Authors - Kathleen M McAndrews View Article
human	WB	PLoS Genet Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors. Authors - Emily M Schleicher KD Validated View Article
human	WB	Biochim Biophys Acta Gene Regul Mech PP2A and its adapter protein IER5 induce the DNA-binding ability and target gene expression of E2F1 via dephosphorylation at serine 375 Authors - Hiroto Takeuchi View Article
mouse	WB,IHC	Bull Exp Biol Med Knockdown of Long Noncoding RNA CCAT2 Suppresses Malignant Phenotype in Human Laryngeal Squamous Cell Carcinoma Authors - M Li View Article

Validation Data Gallery

WB analysis using 22067-1-AP

WB analysis using 22067-1-AP

WB analysis of HEK-293 using 22067-1-AP

IP experiment of HEK-293 using 22067-1-AP

IP experiment of NIH/3T3 using 22067-1-AP

IHC staining of human stomach cancer using 22067-1-AP

IHC staining of human stomach cancer using 22067-1-AP

IHC staining of human testis using 22067-1-AP

IHC staining of human testis using 22067-1-AP

FC experiment of NIH/3T3 using 22067-1-AP

CDK8 Polyclonal antibody

CDK8 Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

CDK8 mediated inflammatory microenvironment aggravates osteoarthritis progression

Loss of MED12 activates the TGFβ pathway to promote chemoresistance and replication fork stability in BRCA-deficient cells.

CDK8 remodels the tumor microenvironment and promotes resistance to KRAS(G12D) inhibitors and daraxonrasib in PDAC.

Dual genome-wide CRISPR knockout and CRISPR activation screens identify mechanisms that regulate the resistance to multiple ATR inhibitors.

PP2A and its adapter protein IER5 induce the DNA-binding ability and target gene expression of E2F1 via dephosphorylation at serine 375

Knockdown of Long Noncoding RNA CCAT2 Suppresses Malignant Phenotype in Human Laryngeal Squamous Cell Carcinoma

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