Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of HepG2 using 27027-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 27027-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 27027-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse embryo using 27027-1-AP
mouse embryo tissue were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 27027-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27027-1-AP (CDK7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 27027-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27027-1-AP (CDK7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 27027-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDK7 antibody (27027-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

CDK7 Polyclonal antibody

CDK7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 27027-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CDKN7, CDK-activating kinase 1, CDK activating kinase, CAK1, CAK

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, MCF-7 cells, mouse embryo tissue
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 6 publications below

Product Information

27027-1-AP targets CDK7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25726 Product name: Recombinant human CDK7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 183-345 aa of BC005298 Sequence: LLFGARMYGVGVDMWAVGCILAELLLRVPFLPGDSDLDQLTRIFETLGTPTEEQWPDMCSLPDYVTFKSFPGIPLHHIFSAAGDDLLDLIQGLFLFNPCARITATQALKMKYFSNRPGPTPGCQLPRPNCPVETLKEQSNPALAIKRKRTEALEQGGLPKKLI 相同性解析による交差性が予測される生物種
Full Name	cyclin-dependent kinase 7
Calculated molecular weight	39 kDa
Observed molecular weight	39 kDa
GenBank accession number	BC005298
Gene Symbol	CDK7
Gene ID (NCBI)	1022
RRID	AB_2880728
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P50613
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for CDK7 antibody 27027-1-AP	Download protocol
IHC protocol for CDK7 antibody 27027-1-AP	Download protocol
WB protocol for CDK7 antibody 27027-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	ACS Nano Nanomaterial-Facilitated Cyclin-Dependent Kinase 7 Inhibition Suppresses Gallbladder Cancer Progression via Targeting Transcriptional Addiction. Authors - Chen-Song Huang View Article
mouse	WB	Genes Dev TRF1 uses a noncanonical function of TFIIH to promote telomere replication Authors - Zhe Yang View Article
human	WB	Front Oncol Identification and validation of a potential key gene SGOL1 for poor prognosis in hepatocellular carcinoma based on a bioinformatics approach Authors - Xiaobin Fei View Article
human	WB	Cell Signal TAGLN2 targeted control of ARPC5-mediated activation of the MEK/ERK signaling pathway influences the proliferation, invasion, and metastasis of pancreatic cancer cells Authors - Yongjia Gao View Article
human,mouse	WB	J Immunother Cancer Adenovirus vaccine targeting kinases induces potent antitumor immunity in solid tumors Authors - Fei Zhu View Article
human	WB	Nat Commun CDK9 recruits HUWE1 to degrade RARα and offers therapeutic opportunities for cutaneous T-cell lymphoma Authors - Chen-Hui Luo View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HepG2 using 27027-1-AP

WB analysis of HepG2 using 27027-1-AP

WB analysis of MCF-7 using 27027-1-AP

WB analysis of mouse embryo using 27027-1-AP

IHC staining of human breast cancer using 27027-1-AP

IHC staining of human breast cancer using 27027-1-AP

IF Staining of HepG2 using 27027-1-AP

CDK7 Polyclonal antibody

CDK7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Nanomaterial-Facilitated Cyclin-Dependent Kinase 7 Inhibition Suppresses Gallbladder Cancer Progression via Targeting Transcriptional Addiction.

TRF1 uses a noncanonical function of TFIIH to promote telomere replication

Identification and validation of a potential key gene SGOL1 for poor prognosis in hepatocellular carcinoma based on a bioinformatics approach

TAGLN2 targeted control of ARPC5-mediated activation of the MEK/ERK signaling pathway influences the proliferation, invasion, and metastasis of pancreatic cancer cells

Adenovirus vaccine targeting kinases induces potent antitumor immunity in solid tumors

CDK9 recruits HUWE1 to degrade RARα and offers therapeutic opportunities for cutaneous T-cell lymphoma