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Species Reactivity
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK7 Polyclonal antibody

CDK7 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27027-1-AP

Synonyms

39 kDa protein kinase, CAK, CAK1, CDK activating kinase, CDK7, CDKN7, Cell division protein kinase 7, cyclin dependent kinase 7, MO15, p39 Mo15, p39MO15, STK1

Validation Data Gallery

Applications:
  • WB analysis of HepG2 using 27027-1-AP

    WB analysis of HepG2 using 27027-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 27027-1-AP

    WB analysis of HepG2 using 27027-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 27027-1-AP

    WB analysis of MCF-7 using 27027-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse embryo using 27027-1-AP

    WB analysis of mouse embryo using 27027-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 27027-1-AP (CDK7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 27027-1-AP

    IHC staining of human breast cancer using 27027-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27027-1-AP (CDK7 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK7 antibody included
  • IHC staining of human breast cancer using 27027-1-AP

    IHC staining of human breast cancer using 27027-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27027-1-AP (CDK7 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDK7 antibody included

Tested Applications

Positive WB detected inHepG2 cells, MCF-7 cells, mouse embryo tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CDK7 Ready-To-Use IHC Kit. CDK7 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

The immunogen of 27027-1-AP is CDK7 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK7 fusion protein Ag25726 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 7
Calculated molecular weight 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC005298
Gene symbol CDK7
Gene ID (NCBI) 1022
RRIDAB_2880728
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CDK7 antibody 27027-1-APDownload protocol
IHC protocol for CDK7 antibody 27027-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

ACS Nano

Nanomaterial-Facilitated Cyclin-Dependent Kinase 7 Inhibition Suppresses Gallbladder Cancer Progression via Targeting Transcriptional Addiction.

Authors - Chen-Song Huang
View Article
mouseWB

Genes Dev

TRF1 uses a noncanonical function of TFIIH to promote telomere replication

Authors - Zhe Yang
View Article
humanWB

Front Oncol

Identification and validation of a potential key gene SGOL1 for poor prognosis in hepatocellular carcinoma based on a bioinformatics approach

Authors - Xiaobin Fei
View Article