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  • Featured Product
  • KD/KO Validated

CDK6 Polyclonal antibody

CDK6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14052-1-AP

Synonyms

CDK6, Cell division protein kinase 6, cyclin dependent kinase 6, PLSTIRE

Validation Data Gallery

Applications:
  • WB analysis using 14052-1-AP

    WB analysis using 14052-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 14052-1-AP

    WB analysis of HeLa using 14052-1-AP

    WB result of CDK6 antibody (14052-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK6 transfected HeLa cells.

  • WB analysis of MOLT-4 using 14052-1-AP

    WB analysis of MOLT-4 using 14052-1-AP

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 14052-1-AP

    WB analysis using 14052-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of C2C12 cells using 14052-1-AP

    WB analysis of C2C12 cells using 14052-1-AP

    C2C12 cells (shRNA samples) were subjected to SDS PAGE followed by western blot with 14052-1-AP (CDK6 antibody) at dilution of 1:500. .

  • IP experiment of Jurkat using 14052-1-AP

    IP experiment of Jurkat using 14052-1-AP

    IP result of anti-CDK6 (IP:14052-1-AP, 4ug; Detection:14052-1-AP 1:1000) with Jurkat cells lysate 2880 ug.

  • IHC staining of human lung cancer using 14052-1-AP

    IHC staining of human lung cancer using 14052-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 14052-1-AP

    IHC staining of human lung cancer using 14052-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human urothelial carcinoma using 14052-1-AP

    IHC staining of human urothelial carcinoma using 14052-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human urothelial carcinoma using 14052-1-AP

    IHC staining of human urothelial carcinoma using 14052-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 14052-1-AP

    IHC staining of rat testis using 14052-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 14052-1-AP

    IHC staining of human endometrial cancer using 14052-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 14052-1-AP

    IHC staining of human endometrial cancer using 14052-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14052-1-AP (CDK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 14052-1-AP

    IF Staining of HeLa using 14052-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 14052-1-AP (CDK6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 14052-1-AP

    FC experiment of HeLa using 14052-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human CDK6 (14052-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, C2C12 cells, HeLa cells, MOLT-4 cells, K-562 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman lung cancer tissue, human endometrial cancer tissue, human urothelial carcinoma tissue, rat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

14052-1-AP targets CDK6 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, goat, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDK6 fusion protein Ag5275 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 6
Calculated molecular weight 326 aa, 36 kDa
Observed molecular weight 36-40 kDa
GenBank accession numberBC027989
Gene symbol CDK6
Gene ID (NCBI) 1021
RRIDAB_10642144
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin-dependent kinase 6 (CDK6) is an enzyme belonging to the CDK family. CDK6 is a catalytic subunit of a 

protein kinase complex that is essential for progression through the G1 phase of the cell cycle and G1/S transition, 

thus activating cell proliferation. CDK6 partners with cyclins to phosphorylate the retinoblastoma (pRb) protein to 

release its binding partner E2F; a transcription factor that drives gene expression necessary for DNA replication 

(PMID: 8114739). CDK6 was long thought of as a redundant homolog of CDK4 as they perform similar roles in cell 

cycle progression. But recently it has become apparent that CDK6 and CDK4 differ in tissue-specific functions, 

where CDK6 plays a role in differentiation (PMID: 16410727) but also in contribution to tumor development.


What is the molecular weight of CDK6?

The molecular weight of CDK6 is approximately 36-40 kDa (PMID: 23563707, 8114739).


What is the subcellular localization of CDK6?

CDK6 is ubiquitously expressed through the nucleus and cytoplasm. However, the most active complexes are 

located in the nuclei of proliferating cells.


What is the expression pattern of CDK6?

CDK6 activity first occurs midway through the G1 phase. Its expression is regulated by D-type cyclins and 

members of the INK4 family of CDK inhibitors (PMID: 7739547).


What is CDK6's role in cancer?

CDK6 is involved in a critical regulatory point in the cell cycle and so is found to be unbalanced in approximately 

80-90% of tumors (PMID: 23356980). Loss of cell cycle control is the initial step in the development of the 

hallmarks of cancer, such as sustained proliferative signaling and dysregulated cellular energetics. Deregulation 

of CDK6 has an important role in lymphoma by increasing angiogenesis (PMID: 23948297) and upregulated CDK6 

expression is also associated with a poor clinical prognosis in medulloblastoma (PMID: 23172372).


Protocols

Product Specific Protocols
WB protocol for CDK6 antibody 14052-1-APDownload protocol
IHC protocol for CDK6 antibody 14052-1-APDownload protocol
IF protocol for CDK6 antibody 14052-1-APDownload protocol
IP protocol for CDK6 antibody 14052-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Cell Biol

miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.

Authors - Cao Jingli J
View Article
humanWB,IHC

Nat Commun

The RNA-binding protein LRPPRC promotes resistance to CDK4/6 inhibition in lung cancer

Authors - Wei Zhou
View Article
humanWB

Nat Commun

ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.

Authors - Rokana Taftaf
View Article
humanWB

Nat Commun

Identification of predictors of drug sensitivity using patient-derived models of esophageal squamous cell carcinoma.

Authors - Dan Su
View Article
humanWB

Cancer Res

CDK4/6 inhibition enhances oncolytic virus efficacy by potentiating tumor-selective cell killing and T cell activation in refractory glioblastoma.

Authors - Deyin Guo
  • KD Validated
View Article
humanWB

Biomaterials

Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.

Authors - Hongyuan Song
View Article