Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
31702-1-AP targets CDK5RAP2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36245 Product name: Recombinant human CDK5RAP2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1221-1571 aa of BC146782 Sequence: MQLFSEIHNLQNKFRDLSPPRYDSLVQSQARELSLQRQQIKDGHGICVISRQHMNTMIKAFEELLQASDVDYCVAEGFQEQLNQCAELLEKLEKLFLNGKSVGVEMNTQNELMERIEEDNLTYQHLLPESPEPSASHALSDYETSEKSFFSRDQKQDNETEKTSVMVNSFSQDLLMEHIQEIRTLRKRLEESIKTNEKLRKQLERQGSEFVQGSTSIFASGSELHSSLTSEIHFLRKQNQALNAMLIKGSRDKQKENDKLRESLSRKTVSLEHLQREYASVKEENERLQKEGSEKERHNQQLIQEVRCSGQELSRVQEELKLRQQLLSQNDKLLQSLRVELKAYEKLDEEH 相同性解析による交差性が予測される生物種 |
| Full Name | CDK5 regulatory subunit associated protein 2 |
| Calculated molecular weight | 1893 aa, 215 kDa |
| Observed molecular weight | 210-215 kDa |
| GenBank accession number | BC146782 |
| Gene Symbol | CDK5RAP2 |
| Gene ID (NCBI) | 55755 |
| RRID | AB_3670087 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96SN8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cyclin-dependent kinase 5 regulatory subunit associated protein 2 (CDK5RAP2) is a pericentriolar matrix (PCM) protein that regulates centriole engagement and centrosome cohesion during the cell cycle. CDK5RAP2 is associated with several cancers, including breast cancer, oral squamous cell carcinoma and haematological malignancies.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDK5RAP2 antibody 31702-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |