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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK4 Recombinant antibody (83994-3-RR)

    WB analysis using 83994-3-RR (same clone as 83994-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83994-3-RR (CDK4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CDK4 Recombinant antibody (83994-3-RR)

    WB analysis of HeLa using 83994-3-RR (same clone as 83994-3-PBS)

    WB result of CDK4 antibody (83994-3-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-CDK4 transfected HeLa cells. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using CDK4 Recombinant antibody (83994-3-RR)

    IP experiment of HeLa using 83994-3-RR (same clone as 83994-3-PBS)

    IP result of anti-CDK4 (IP:83994-3-RR, 4ug; Detection:83994-3-RR 1:500) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical squamous cancer tissue using CDK4 Recombinant antibody (83994-3-RR)

    IHC staining of human cervical squamous cancer using 83994-3-RR (same clone as 83994-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical squamous cancer tissue slide using 83994-3-RR (CDK4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CDK4 Recombinant antibody (83994-3-RR)

    IF Staining of MCF-7 using 83994-3-RR (same clone as 83994-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CDK4 antibody (83994-3-RR, Clone: 241053F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83994-3-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CDK4 Recombinant monoclonal antibody, PBS Only

CDK4 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 83994-3-PBS
Clone No.241053F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

241053F10, Cell division protein kinase 4, CMM3, cyclin dependent kinase 4, Cyclin-dependent kinase 4

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83994-3-PBS targets CDK4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag20538

Product name: Recombinant human CDK4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-303 aa of BC010153

Sequence: MATSRYEPVAEIGVGAYGTVYKARDPHSGHFVALKSVRVPNGGGGGGGLPISTVREVALLRRLEAFEHPNVVRLMDVCATSRTDREIKVTLVFEHVDQDLRTYLDKAPPPGLPAETIKDLMRQFLRGLDFLHANCIVHRDLKPENILVTSGGTVKLADFGLARIYSYQMALTPVVVTLWYRAPEVLLQSTYATPVDMWSVGCIFAEMFRRKPLFCGNSEADQLGKIFDLIGLPPEDDWPRDVSLPRGAFPPRGPRPVQSVVPEMEESGAQLLLEMLTFNPHKRISAFRALQHSYLHKDEGNPE

相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 4
Calculated molecular weight 34 kDa
Observed molecular weight30-34 kDa
GenBank accession numberBC010153
Gene Symbol CDK4
Gene ID (NCBI) 1019
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP11802
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1-to S-phase transition during the cell cycle, and its expression is primarily controlled at the transcriptional level(PMID:17253961). The CCND1-CDK4 axis is critical not only in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment, which are vital to sustain tumor outgrowth (PMID:21844184).

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