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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CDK4 Monoclonal antibody, PBS Only

CDK4 Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G2C12

Cat no : 66950-1-PBS

Synonyms

CDK4, Cell division protein kinase 4, CMM3, cyclin dependent kinase 4, PSK J3

Validation Data Gallery

Applications:
  • WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • WB analysis of 4T1 using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis of 4T1 using 66950-1-Ig (same clone as 66950-1-PBS)

    4T1 cells were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis using 66950-1-Ig (same clone as 66950-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis of MCF-7 using 66950-1-Ig (same clone as 66950-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis of HeLa using 66950-1-Ig (same clone as 66950-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66950-1-Ig (same clone as 66950-1-PBS)

    WB analysis of HEK-293 using 66950-1-Ig (same clone as 66950-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66950-1-Ig (CDK4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66950-1-Ig (CDK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK4 antibody included
  • IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    IHC staining of human breast cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66950-1-Ig (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK4 antibody included
  • IHC staining of human lung cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    IHC staining of human lung cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66950-1-Ig (CDK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK4 antibody included
  • IHC staining of human lung cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    IHC staining of human lung cancer using 66950-1-Ig (same clone as 66950-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66950-1-Ig (CDK4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CDK4 antibody included
  • FC experiment of MCF-7 using 66950-1-Ig (same clone as 66950-1-PBS)

    FC experiment of MCF-7 using 66950-1-Ig (same clone as 66950-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human CDK4 (66950-1-Ig, Clone:1G2C12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66950-1-PBS in a different storage buffer formulation.

Tested Applications

Planning an IHC experiment? We recommend our IHCeasy CDK4 Ready-To-Use IHC Kit. CDK4 primary antibody included.

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Product Information

66950-1-PBS targets CDK4 in WB, FC, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CDK4 fusion protein Ag20538 相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 4
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC010153
Gene symbol CDK4
Gene ID (NCBI) 1019
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. It is essential for the G1- to S-phase transition during the cell cycle and its expression is primarily controlled at the transcriptional level(PMID:17253961). CCND1-CDK4 axis is not only critical in glial tumor cells but also in stromal-derived cells in the surrounding tumor microenvironment that are vital to sustain tumor outgrowth(PMID:21844184).