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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDK3 Polyclonal antibody (55103-1-AP)

    WB analysis using 55103-1-AP (same clone as 55103-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CDK3 Polyclonal antibody (55103-1-AP)

    WB analysis of MCF-7 using 55103-1-AP (same clone as 55103-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using CDK3 Polyclonal antibody (55103-1-AP)

    WB analysis of MCF-7 using 55103-1-AP (same clone as 55103-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using CDK3 Polyclonal antibody (55103-1-AP)

    WB analysis of Jurkat using 55103-1-AP (same clone as 55103-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55103-1-AP (CDK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CDK3 Polyclonal antibody (55103-1-AP)

    IHC staining of human breast cancer using 55103-1-AP (same clone as 55103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55103-1-AP (CDK3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CDK3 Polyclonal antibody (55103-1-AP)

    IHC staining of human breast cancer using 55103-1-AP (same clone as 55103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55103-1-AP (CDK3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK3 Polyclonal antibody (55103-1-AP)

    IF Staining of HeLa using 55103-1-AP (same clone as 55103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK3 antibody (55103-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using CDK3 Polyclonal antibody (55103-1-AP)

    FC experiment of HeLa using 55103-1-AP (same clone as 55103-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDK3 Polyclonal antibody (55103-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 55103-1-PBS in a different storage buffer formulation.

CDK3 Polyclonal antibody, PBS Only

CDK3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 55103-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

CDKN3, EC:2.7.11.22, Cyclin-dependent kinase 3, Cyclin dependent kinase 3, Cell division protein kinase 3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55103-1-PBS targets CDK3 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 3
Calculated molecular weight 35 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberNM_001258
Gene Symbol CDK3
Gene ID (NCBI) 1018
RRIDAB_10804352
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00526
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CDK3 belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. It is probably involved in the control of the cell cycle. CDK3 interacts with a yet unknown type of cyclin. It can phosphorylate histone H1. Endogenous Cyclin C/Cdk3 complexes are critical to promote exit from quiescence in an Rb-dependent manner. Cyclin C/Cdk3 complexes is a key regulators of cell cycle reentry in human cells. (PMID: 15130482 )The antibody is specific to CDK3.

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