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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CDK2AP1 Polyclonal antibody (13060-2-AP)

    WB analysis of HEK-293 using 13060-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CDK2AP1 Polyclonal antibody (13060-2-AP)

    WB analysis of HEK-293 using 13060-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CDK2AP1 Polyclonal antibody (13060-2-AP)

    WB analysis of HeLa using 13060-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CDK2AP1 Polyclonal antibody (13060-2-AP)

    WB analysis of HeLa using 13060-2-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13060-2-AP (CDK2AP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human placenta using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human placenta using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human heart using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human heart using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human placenta using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human placenta using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human skin using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human skin using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human brain using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human brain using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human liver using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human liver using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human spleen using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human spleen using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human ovary using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IHC staining of human ovary using 13060-2-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13060-2-AP (CDK2AP1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK2AP1 Polyclonal antibody (13060-2-AP)

    IF Staining of HeLa using 13060-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2AP1 antibody (13060-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

CDK2AP1 Polyclonal antibody

CDK2AP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13060-2-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CDK2 associated protein 1, CDK2-associated protein 1, CDKAP1, Cyclin-dependent kinase 2-associated protein 1, Deleted in oral cancer 1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman placenta tissue, human brain tissue, human heart tissue, human liver tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13060-2-AP targets CDK2AP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3736

Product name: Recombinant human CDK2AP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-115 aa of BC034717

Sequence: MSYKPNLAAHMPAAALNAAGSVHSPSTSMATSSQYRQLLSDYGPPSLGYTQGTGNSQVPQSKYAELLAIIEELGKEIRPTYAGSKSAMERLKRGIIHARGLVRECLAETERNARS

相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 2 associated protein 1
Calculated molecular weight 115 aa, 13 kDa
Observed molecular weight 12 kDa
GenBank accession numberBC034717
Gene Symbol CDK2AP1
Gene ID (NCBI) 8099
RRIDAB_10340136
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO14519
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CDK2AP1 antibody 13060-2-APDownload protocol
IHC protocol for CDK2AP1 antibody 13060-2-APDownload protocol
WB protocol for CDK2AP1 antibody 13060-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

Cathepsin L interacts with CDK2-AP1 as a potential predictor of prognosis in patients with breast cancer.

Authors - Zhan Wang
View Article
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