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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using CDK2 Monoclonal antibody (60312-1-Ig)

    WB analysis of K-562 using 60312-1-Ig (same clone as 60312-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 60312-1-Ig (CDK2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CDK2 Monoclonal antibody (60312-1-Ig)

    IHC staining of human cervical cancer using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60312-1-Ig (CDK2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CDK2 Monoclonal antibody (60312-1-Ig)

    IHC staining of human cervical cancer using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 60312-1-Ig (CDK2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CDK2 Monoclonal antibody (60312-1-Ig)

    IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60312-1-Ig (CDK2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using CDK2 Monoclonal antibody (60312-1-Ig)

    IHC staining of human lymphoma using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 60312-1-Ig (CDK2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDK2 Monoclonal antibody (60312-1-Ig)

    IF Staining of HeLa using 60312-1-Ig (same clone as 60312-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDK2 antibody (60312-1-Ig, Clone: 4E2E10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using CDK2 Monoclonal antibody (60312-1-Ig)

    FC experiment of HepG2 using 60312-1-Ig (same clone as 60312-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug CDK2 Monoclonal antibody (60312-1-Ig, Clone:4E2E10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60312-1-PBS in a different storage buffer formulation.

CDK2 Monoclonal antibody, PBS Only

CDK2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 60312-1-PBS
Clone No.4E2E10

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

4E2E10, CDKN2, Cell division protein kinase 2, Cyclin dependent kinase 2, Cyclin-dependent kinase 2

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60312-1-PBS targets CDK2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag21310

Product name: Recombinant human CDK2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 8-210 aa of BC003065

Sequence: EKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIREISLLKELNHPNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGIPLPLIKSYLFQLLQGLAFCHSHRVLHRDLKPQNLLINTEGAIKLADFGLARAFGVPVRTYTHEVVTLWYRAPEILLGCKYYSTAVDIWSLGCIFAEMVTRRALFPGDSEID

相同性解析による交差性が予測される生物種
Full Name cyclin-dependent kinase 2
Calculated molecular weight 33 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC003065
Gene Symbol CDK2
Gene ID (NCBI) 1017
RRIDAB_2881424
Conjugate Unconjugated
Form
FormLiquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
UNIPROT IDP24941
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CDK2(Cyclin-dependent kinase 2) is also named as CDKN2 and belongs to the protein kinase superfamily,CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily.It is involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis.It has 2 isoforms produced by alternative splicing.

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