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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using P-cadherin Monoclonal antibody (66295-1-Ig)

    WB analysis of A431 using 66295-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 66295-1-Ig (P-cadherin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using P-cadherin Monoclonal antibody (66295-1-Ig)

    IHC staining of human placenta using 66295-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using P-cadherin Monoclonal antibody (66295-1-Ig)

    IHC staining of human placenta using 66295-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66295-1-Ig (P-cadherin antibody) at dilution of 1:250 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

P-cadherin Monoclonal antibody

P-cadherin Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66295-1-Ig
Clone No.1G10F7
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

Cadherin 3, CDH3, CDHP, HJMD, P cadherin, PCAD, P-cadherin, Placental cadherin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66295-1-Ig targets P-cadherin in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag4758

Product name: Recombinant human CDH3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-349 aa of BC041846

Sequence: SEPCRAVFREAEVTLEAGGAEQEPGQALGKVFMGCPGQEPALFSTDNDDFTVRNGETVQERRSLKERNPLKIFPSKRILRRHKRDWVVAPISVPENGKGPFPQRLNQLKSNKDRDTKIFYSITGPGADSPPEGVFAVEKETGWLLLNKPLDREEIAKYELFGHAVSENGASVEDPMNISIIVTDQNDHKPKFTQDTFRGSVLEGVLPGTSVMQMTATDEDDAIYTYNGVVAYSIHSQEPKDPHDLMFTIHRSTGTISVISSGLDREKVPEYTLTIQATDMDGDGSTTTAVAVVEILDANDNAPMFDPQKYEAHVPENAVGHEVQRL

相同性解析による交差性が予測される生物種
Full Name cadherin 3, type 1, P-cadherin (placental)
Calculated molecular weight 829 aa, 91 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC041846
Gene Symbol P-cadherin
Gene ID (NCBI) 1001
RRIDAB_2881678
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDP22223
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.

Protocols

Product Specific Protocols
WB protocol for P-cadherin antibody 66295-1-IgDownload protocol
IHC protocol for P-cadherin antibody 66295-1-IgDownload protocol
FC protocol for P-cadherin antibody 66295-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Biosens Bioelectron

Constructing a highly sensitive duplex immunoassay using AuNPs and AgNPs as nanolabels for investigating the epithelial-mesenchymal transition occurring on circulating tumor cells with lung cancer patients

Authors - Yunuo Dai
View Article