Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of A431 using 13773-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 13773-1-AP (P-cadherin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of A431 using 13773-1-AP
IP result of anti-P-cadherin (IP:13773-1-AP, 4ug; Detection:13773-1-AP 1:300) with A431 cells lysate 3040ug.
IHC staining of human stomach cancer using 13773-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 13773-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13773-1-AP (P-cadherin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 13773-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 13773-1-AP (P-cadherin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of A431 using 13773-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using P-cadherin antibody (13773-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of A431 using 13773-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 13773-1-AP (P-cadherin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

P-cadherin Polyclonal antibody

P-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 13773-1-AP

Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Cadherin 3, Cadherin-3, CDH3, CDHP, HJMD

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (16)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells
Positive IP detected in	A431 cells
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:300-1:1200
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 9 publications below
IHC	See 6 publications below
IF	See 3 publications below

Product Information

13773-1-AP targets P-cadherin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4758 Product name: Recombinant human CDH3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-349 aa of BC041846 Sequence: SEPCRAVFREAEVTLEAGGAEQEPGQALGKVFMGCPGQEPALFSTDNDDFTVRNGETVQERRSLKERNPLKIFPSKRILRRHKRDWVVAPISVPENGKGPFPQRLNQLKSNKDRDTKIFYSITGPGADSPPEGVFAVEKETGWLLLNKPLDREEIAKYELFGHAVSENGASVEDPMNISIIVTDQNDHKPKFTQDTFRGSVLEGVLPGTSVMQMTATDEDDAIYTYNGVVAYSIHSQEPKDPHDLMFTIHRSTGTISVISSGLDREKVPEYTLTIQATDMDGDGSTTTAVAVVEILDANDNAPMFDPQKYEAHVPENAVGHEVQRL 相同性解析による交差性が予測される生物種
Full Name	cadherin 3, type 1, P-cadherin (placental)
Calculated molecular weight	829 aa, 91 kDa
Observed molecular weight	120 kDa
GenBank accession number	BC041846
Gene Symbol	P-cadherin
Gene ID (NCBI)	1001
RRID	AB_10596468
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P22223
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. P-cadherin (placental cadherin), also known as CDH3 (cadherin 3), is a classical member of the cadherin superfamily which also include E-, N-, R-, and B-cadherins. Aberrant expression of P-cadherin has been reported in many cancers, including breast, gastric, endometrial, ovarian, and prostate cancer.

Protocols

Product Specific Protocols
IF protocol for P-cadherin antibody 13773-1-AP	Download protocol
IHC protocol for P-cadherin antibody 13773-1-AP	Download protocol
IP protocol for P-cadherin antibody 13773-1-AP	Download protocol
WB protocol for P-cadherin antibody 13773-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Nat Commun Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice. Authors - Hans I-Chen Harn View Article
mouse	IF	Mol Ther Nucleic Acids Inhibition of the lncRNA MIAT prevents podocyte injury and mitotic catastrophe in diabetic nephropathy. Authors - Ziyang Wang View Article
human	IHC	Aging (Albany NY) Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism. Authors - Jing Han View Article
human	WB,IHC	Front Oncol Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer. Authors - Meng-Jie Shan View Article
human	WB,IHC	Int J Biol Sci KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling. Authors - Li Li View Article
human	IHC	J Oral Pathol Med Basal differentiation and expression status of SOX2 and KLF4 in basal layers are prognostic factors for disease-specific survival in oral squamous cell carcinoma Authors - Kazuhiro Murakami View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A431 using 13773-1-AP

IP experiment of A431 using 13773-1-AP

IHC staining of human stomach cancer using 13773-1-AP

IHC staining of human stomach cancer using 13773-1-AP

IF Staining of A431 using 13773-1-AP

IF Staining of A431 using 13773-1-AP

IF Staining of A431 using 13773-1-AP

P-cadherin Polyclonal antibody

P-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration of laboratory and spiny mice.

Inhibition of the lncRNA MIAT prevents podocyte injury and mitotic catastrophe in diabetic nephropathy.

Transcriptional dysregulation of TRIM29 promotes colorectal cancer carcinogenesis via pyruvate kinase-mediated glucose metabolism.

Screening and Identification of Key Biomarkers of Gastric Cancer: Three Genes Jointly Predict Gastric Cancer.

KLF4-Mediated CDH3 Upregulation Suppresses Human Hepatoma Cell Growth and Migration via GSK-3β Signaling.

Basal differentiation and expression status of SOX2 and KLF4 in basal layers are prognostic factors for disease-specific survival in oral squamous cell carcinoma