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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDH26 Recombinant antibody (84143-1-RR)

    WB analysis using 84143-1-RR (same clone as 84143-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84143-1-RR (CDH26 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84143-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-2 OS using CDH26 Recombinant antibody (84143-1-RR)

    FC experiment of U-2 OS using 84143-1-RR (same clone as 84143-1-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug CDH26 Recombinant antibody (84143-1-RR, Clone:240934F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84143-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using CDH26 Recombinant antibody (84143-1-RR)

    FC experiment of HeLa using 84143-1-RR (same clone as 84143-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug CDH26 Recombinant antibody (84143-1-RR, Clone:240934F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84143-1-PBS in a different storage buffer formulation.

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CDH26 Recombinant antibody, PBS Only

CDH26 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84143-1-PBS
Clone No.240934F9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Cadherin-like protein VR20, Cadherin-like protein 26, 240934F9

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84143-1-PBS targets CDH26 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cadherin-like 26
Calculated molecular weight 95 kDa
Observed molecular weight18 kDa
GenBank accession numberNM_021810
Gene Symbol CDH26
Gene ID (NCBI) 60437
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IXH8
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CDH26, also named VR20, is a member of cadherins which are calcium-dependent cell adhesion proteins. Cadherins preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. CDH26 produces 4 isoforms through alternative splicing, with molecular weights of 13, 18, 92, and 95 kDa, respectively.