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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDH26 Polyclonal antibody (20057-1-AP)

    WB analysis using 20057-1-AP (same clone as 20057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20057-1-AP (CDH26 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20057-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CDH26 Polyclonal antibody (20057-1-AP)

    WB analysis using 20057-1-AP (same clone as 20057-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20057-1-AP (CDH26 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20057-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using CDH26 Polyclonal antibody (20057-1-AP)

    IF Staining of mouse liver using 20057-1-AP (same clone as 20057-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using CDH26 antibody (20057-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20057-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CDH26 Polyclonal antibody (20057-1-AP)

    IF Staining of MCF-7 using 20057-1-AP (same clone as 20057-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CDH26 antibody (20057-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20057-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CDH26 Polyclonal antibody, PBS Only

CDH26 Polyclonal Antibody for WB, IF/ICC, IF-P, Indirect ELISA
Cat No. 20057-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IF-P, Indirect ELISA

Synonyms

Cadherin-like protein VR20, Cadherin-like protein 26

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20057-1-PBS targets CDH26 in WB, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name cadherin-like 26
Calculated molecular weight 95 kDa
Observed molecular weight18-22 kDa, 95-100 kDa
GenBank accession numberNM_021810
Gene Symbol CDH26
Gene ID (NCBI) 60437
RRIDAB_2878637
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IXH8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

CDH26, also named VR20, is a member of cadherins which are calcium-dependent cell adhesion proteins. Cadherins preferentially interact with themselves in a homophilic manner in connecting cells. Cadherins may thus contribute to the sorting of heterogeneous cell types. CDH26 produces 4 isoforms through alternative splicing, with molecular weights of 13, 18, 92, and 95 kDa, respectively.

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