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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse colon tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    WB analysis of mouse colon using 83793-5-RR (same clone as 83793-5-PBS)

    mouse colon tissue was subjected to SDS PAGE followed by western blot with 83793-5-RR (Cadherin-17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83793-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    IHC staining of mouse colon using 83793-5-RR (same clone as 83793-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83793-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    IHC staining of mouse colon using 83793-5-RR (same clone as 83793-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83793-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    IHC staining of human stomach cancer using 83793-5-RR (same clone as 83793-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83793-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    IHC staining of human stomach cancer using 83793-5-RR (same clone as 83793-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83793-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    IHC staining of human colon cancer using 83793-5-RR (same clone as 83793-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83793-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    IHC staining of human colon cancer using 83793-5-RR (same clone as 83793-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83793-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human stomach cancer tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    IF Staining of human stomach cancer using 83793-5-RR (same clone as 83793-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using Cadherin-17 antibody (83793-5-RR, Clone: 240681B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83793-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human stomach cancer tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    IF Staining of human stomach cancer using 83793-5-RR (same clone as 83793-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using Cadherin-17 antibody (83793-5-RR, Clone: 240681B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83793-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human stomach cancer tissue using Cadherin-17 Recombinant antibody (83793-5-RR)

    IF Staining of human stomach cancer using 83793-5-RR (same clone as 83793-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human stomach cancer tissue using Cadherin-17 antibody (83793-5-RR, Clone: 240681B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83793-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83793-5-RR

    Affinity and Kinetic Characterization of 83793-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83793-5-RR against Human Cadherin-17 were performed. The affinity constant is below 1 pM.

Cadherin-17 Recombinant monoclonal antibody, PBS Only

Cadherin-17 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83793-5-PBS
Clone No.240681B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Synonyms

CDH17, HPT1, HPT 1, CDH16, Cadherin 17

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83793-5-PBS targets Cadherin-17 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19488

Product name: Recombinant human CDH17 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 89-406 aa of BC113464

Sequence: RSTHNLQVAALDANGIIVEGPVPITIEVKDINDNRPTFLQSKYEGSVRQNSRPGKPFLYVNATDLDDPATPNGQLYYQIVIQLPMINNVMYFQINNKTGAISLTREGSQELNPAKNPSYNLVISVKDMGGQSENSFSDTTSVDIIVTENIWKAPKPVEMVENSTDPHPIKITQVRWNDPGAQYSLVDKEKLPRFPFSIDQEGDIYVTQPLDREEKDAYVFYAVAKDEYGKPLSYPLEIHVKVKDINDNPPTCPSPVTVFEVQENERLGNSIGTLTAHDRDEENTANSFLNYRIVEQTPKLPMDGLFLIQTYAGMLQLA

相同性解析による交差性が予測される生物種
Full Name cadherin 17, LI cadherin (liver-intestine)
Calculated molecular weight 832 aa, 92 kDa
Observed molecular weight120 kDa
GenBank accession numberBC113464
Gene Symbol Cadherin-17
Gene ID (NCBI) 1015
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ12864
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cadherin-17, also known as LI-cadherin, is a member of the cadherin superfamily. Cadherins are transmembrane proteins that mediates cell-cell adhesion, plays important roles during embryogenesis, and is crucial for tissue morphogenesis and maintenance. Cadherin-17 is a component of the gastrointestinal tract and pancreatic ducts and is involved in intestinal peptide transport. It may also play a role in the morphological organization of liver and intestine.