Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
87514-4-PBS targets CDCP1/CD318 in WB, Indirect ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg6199 Product name: Recombinant mouse CD318/CDCP1 protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 30-666 aa of NM_133974.3 Sequence: SEIALPQRSGVTVSIKLGNPALPVKICYIVMSRQHITELIIRPGERKSFTFSCSNPEKHFVLKIEKNIDCMSGPCPFGEVHLQPSTSELPILNRTFIWDVRAHKSIGLELQFATPRLRQIGPGESCADGVTHSISGHIDATEVRIGTFCSNGTVSRIKMQEGVKMALHLPWFHRRNVSGFSIANRSSIKRLCIIESVFEGEGSATLMSANYPGGFPEDELMTWQFVVPAHLRASVSFLNFNVSNCERKEERVEYYIPGSTTNPEVFRLEDKQPGNMAGNFNLSLQGCDQDAQSPGILRLQFQVLVQRPQDESNKTYMVDLSRERTMSLTIEPRPVKHGRRFVPGCFVCLESRTCSTNVTLTAGSIHKISFLCDDLTRLWVNVEKTLSCLDHRYCYRQSFKLQVPDYILQLPVQLHDFSWKLLVPKDKLSLMLVPGQKLQQHTQERPCNTSFGYHVTSTTPGQDLYFGSFCSGGSIEKIQVKQNSSVTLRAYAPSFQQEVSKQGLIVSYTPYFKEEGIFTVTPDTKNKVYLRSPNWDRGLPALSSVSWNISVPSNQVACLTVLKERSGLACQSGRAFMIIQEQQSRAEEIFSLEEEVLPKPSFHHHSFWVNISNCSPMNGKQLDLLFWVTLTPRTVDL 相同性解析による交差性が予測される生物種 |
| Full Name | CUB domain containing protein 1 |
| Calculated molecular weight | 93 kDa |
| Observed molecular weight | 130-140 kDa |
| GenBank accession number | NM_133974.3 |
| Gene Symbol | Cdcp1 |
| Gene ID (NCBI) | 109332 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5U462 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
CD318 (also known as CDCP1, TRASK, SIMA135, or gp140) is a cell-surface glycoprotein with an apparent molecular mass of ∼140 kDa. It consists of three extracellular CUB domains, a transmembrane domain, and an intracellular domain. CD318 can be proteolytically cleaved between the two distal CUB domains by certain serine proteases, resulting in different ratios of the ∼140-kDa intact molecule and the ∼80-kDa cleaved product on various cells. Cleaved CD318 is phosphorylated and activated by Src kinase, then the activated CD318 forms a complex with activated β1 integrin and activates FAK/PI3K/Akt motility signaling to promote early tumor dissemination. Under normal conditions, CD318 is present on many epithelial cells, some hematopoietic cells (18), and mesenchymal stem cells. CD318 is also present on many tumor cells. Up-regulation of CD318 expression is associated with a poor prognosis for many cancer patients.