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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDCA7L Recombinant antibody (83682-4-RR)

    WB analysis using 83682-4-RR (same clone as 83682-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83682-4-RR (CDCA7L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83682-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using CDCA7L Recombinant antibody (83682-4-RR)

    FC experiment of A431 using 83682-4-RR (same clone as 83682-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug CDCA7L Recombinant antibody (83682-4-RR, Clone:240639A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83682-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83682-4-RR

    Affinity and Kinetic Characterization of 83682-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83682-4-RR against Human CDCA7L were performed. The affinity constant is 7.75 nM.

CDCA7L Recombinant monoclonal antibody, PBS Only

CDCA7L Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83682-4-PBS
Clone No.240639A3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

R1, Protein JPO2, JPO2, HR1, Cell division cycle-associated 7-like protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83682-4-PBS targets CDCA7L in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag10944

Product name: Recombinant human CDCA7L protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-269 aa of BC009352

Sequence: MELATRYQIPKEVADIFNAPSDDEEFVGFRDDVPMETLSSEESCDSFDSLESGKQQDVRFHSKYFTEELRRIFIEDTDSETEDFAGFTQSDLNGKTNPEVMVVESDLSDDGKASLVSEEEEDEEEDKATPRRSRSRRSSIGLRVAFQFPTKKLANKPDKNSSSEQLFSSARLQNEKKTILERKKDCRQVIQREDSTSESEDDSRDESQESSDALLKRTMNIKENKAMLAQLLAELNSMPDFFPVRTPTSASRKKTVRRAFSEGQITRRM

相同性解析による交差性が予測される生物種
Full Name cell division cycle associated 7-like
Calculated molecular weight 454 aa, 52 kDa
GenBank accession numberBC009352
Gene Symbol CDCA7L
Gene ID (NCBI) 55536
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ96GN5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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