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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CDCA5 Polyclonal antibody (29316-1-AP)

    WB analysis using 29316-1-AP (same clone as 29316-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29316-1-AP (CDCA5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29316-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CDCA5 Polyclonal antibody (29316-1-AP)

    IHC staining of human skin cancer using 29316-1-AP (same clone as 29316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29316-1-AP (CDCA5 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29316-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CDCA5 Polyclonal antibody (29316-1-AP)

    IHC staining of human skin cancer using 29316-1-AP (same clone as 29316-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 29316-1-AP (CDCA5 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29316-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CDCA5 Polyclonal antibody (29316-1-AP)

    IF Staining of HeLa using 29316-1-AP (same clone as 29316-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CDCA5 antibody (29316-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 29316-1-PBS in a different storage buffer formulation.

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CDCA5 Polyclonal antibody, PBS Only

CDCA5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29316-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Sororin, p35, Cell division cycle-associated protein 5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29316-1-PBS targets CDCA5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDCA5 fusion protein Ag30966 相同性解析による交差性が予測される生物種
Full Name cell division cycle associated 5
Calculated molecular weight 27 kDa
Observed molecular weight27~35 kDa
GenBank accession numberBC011000
Gene Symbol CDCA5
Gene ID (NCBI) 113130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96FF9
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Human cell division cycle associated 5 (CDCA5), also known as Sororin, is required for stable binding of cohesin to chromatid in the S and G2/M phases and degraded through anaphase-promoting complex-dependent ubiquitination in the G0/G1 phase (PMID: 30808873). Suppression of CDCA5 expression with siRNAs inhibited the growth of lung cancer cells. CDCA5 positivity is an independent prognostic factor for lung cancer patients (PMID: 20551060). The predicted molecular size of CDCA is 27 kd, with an electrophoretic mobility of ~35 kd on SDS-PAGE, possibly due to its isoelectric point (pI) of approximately 10 (PMID: 22580470).