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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using CDCA4 Polyclonal antibody (11625-1-AP)

    WB analysis of HeLa using 11625-1-AP (same clone as 11625-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CDCA4 Polyclonal antibody (11625-1-AP)

    WB analysis using 11625-1-AP (same clone as 11625-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CDCA4 Polyclonal antibody (11625-1-AP)

    WB analysis of HeLa using 11625-1-AP (same clone as 11625-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11625-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CDCA4 Polyclonal antibody (11625-1-AP)

    WB analysis of HEK-293 using 11625-1-AP (same clone as 11625-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11625-1-AP (CDCA4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11625-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CDCA4 Polyclonal antibody, PBS Only

CDCA4 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 11625-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11625-1-PBS targets CDCA4 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2214

Product name: Recombinant human CDCA4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-241 aa of BC025263

Sequence: MFARGLKRKCVGHEEDVEGALAGLKTVSSYSLQRQSLLDMSLVKLQLCHMLVEPNLCRSVLIANTVRQIQEEMTQDGTWRTVAPQAAERAPLNRLVSTEILCRAAWGQEGAHPAPGLGDGHTQGPVSDLCPVTSAQAPRHLQSSAWEMDGPRENRGSFHKSLDQIFETLETKNPSCMEELFSDVDSPYYDLDTVLTGMMGGARPGPCEGLEGLAPATPGPSSSCKSDLGELDHVVEILVET

相同性解析による交差性が予測される生物種
Full Name cell division cycle associated 4
Calculated molecular weight 241 aa, 26 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC025263
Gene Symbol CDCA4
Gene ID (NCBI) 55038
RRIDAB_2260414
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXL8
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

TRIP-Br proteins are a set of proteins with the potential to function in both transcriptional control and cell cycle regulation [PMID:18572021]. CECA4 is a member of TRIP-br family, based on its homologous sequence motifs including SERTA and a PHD-bromo-binding site which interacts with a series of bromodomain containing transcriptional cofactors[PMID:17141982]. When activated by E2F, CDCA4 consequently functions as a suppressor of E2F-responsive promoters to participate the regulation of cell proliferation through the E2F/pRb pathway [PMID:16098148].

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