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- KD/KO Validated
CDCA3 Polyclonal antibody
CDCA3 Polyclonal Antibody for IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 15594-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IHC detected in | human breast cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
15594-1-AP targets CDCA3 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CDCA3 fusion protein Ag7951 相同性解析による交差性が予測される生物種 |
| Full Name | cell division cycle associated 3 |
| Calculated molecular weight | 29 kDa |
| GenBank accession number | BC002551 |
| Gene symbol | CDCA3 |
| Gene ID (NCBI) | 83461 |
| RRID | AB_2878154 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDCA3, also named C8, GRCC8, and TOME1, is an F-box-like protein required for entry into mitosis. It acts by participating in E3 ligase complexes that mediate the ubiquitination and degradation of WEE1 kinase at the G2/M phase.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CDCA3 antibody 15594-1-AP | Download protocol |
| IF protocol for CDCA3 antibody 15594-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogene CPSF4 regulates circRNA formation and microRNA mediated gene silencing in hepatocellular carcinoma. | ||
Cancer Cell Int The inhibitory effect of silencing CDCA3 on migration and proliferation in bladder urothelial carcinoma.
| ||
Naunyn Schmiedebergs Arch Pharmacol Curcumin targets miR-134-5p to suppress the progression of colorectal cancer through regulating the CDCA3/CDK1 pathway | ||
Biochem Biophys Res Commun CDCA3 promotes cell proliferation by activating the NF-κB/cyclin D1 signaling pathway in colorectal cancer.
| ||
Pathol Res Pract miR-145-5p suppresses proliferation, metastasis and EMT of colorectal cancer by targeting CDCA3. | ||
J Oncol CDCA3 Predicts Poor Prognosis and Affects CD8+ T Cell Infiltration in Renal Cell Carcinoma |
