• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Use Able AI chat for product recommendations

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CDCA2 Recombinant monoclonal antibody (86379-1-RR)

    WB analysis using 86379-1-RR (same clone as 86379-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86379-1-RR (CDCA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Hepatocellular carcinoma tissue using CDCA2 Recombinant monoclonal antibody (86379-1-RR)

    IHC staining of Hepatocellular carcinoma using 86379-1-RR (same clone as 86379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma tissue slide using 86379-1-RR (CDCA2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of Hepatocellular carcinoma tissue using CDCA2 Recombinant monoclonal antibody (86379-1-RR)

    IHC staining of Hepatocellular carcinoma using 86379-1-RR (same clone as 86379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded Hepatocellular carcinoma tissue slide using 86379-1-RR (CDCA2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CDCA2 Recombinant monoclonal antibody (86379-1-RR)

    IF Staining of HepG2 using 86379-1-RR (same clone as 86379-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDCA2 antibody (86379-1-RR, Clone: 250980D8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86379-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86379-1-RR

    Affinity and Kinetic Characterization of 86379-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86379-1-RR against Human CDCA2 were performed. The affinity constant is below 1 pM.

CDCA2 Recombinant monoclonal antibody, PBS Only

CDCA2 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86379-1-PBS
Clone No.250980D8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Cell division cycle-associated protein 2, Repo Man, Repo-Man

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86379-1-PBS targets CDCA2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag11967

Product name: Recombinant human CDCA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 512-863 aa of BC036214

Sequence: ATSDEDPNTNIMNINENKNIPKAKNKSESENEPKAGTDSPVSCASITEERVASDSPKPALTLQQGQEFSAGGQNAENLCQFFKISPDLNIKCERKDDFLGAAEGKLQCNRLMPNSQKDCHCLGDVLIENTKESKSQSEDLGRKPMESSSVVSCRDRKDRRRSMCYSDGRSLHLEKNGNHTPSSSVGSSVEISLENSELFKDLSDAIEQTFQRRNSETKVRRSTRLQKDLENEGLVWISLPLPSTSQKAKRRTICTFDSSGFESMSPIKETVSSRQKPQMAPPVSDPENSQGPAAGSSDEPGKRRKSFCISTLANTKATSQFKGYRRRSSLNGKGESSLTALERIEHNGERKQ

相同性解析による交差性が予測される生物種
Full Name cell division cycle associated 2
Calculated molecular weight113 kDa
Observed molecular weight140 kDa
GenBank accession numberBC036214
Gene Symbol CDCA2
Gene ID (NCBI) 157313
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ69YH5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Cell division cycle associated 2(CDCA2) is a nuclear protein that is a specific regulatory subunit for protein phosphatase 1γ (PP1γ). CDCA2 is a candidate factor involved in preparing mitotic chromatin for the transition to interphase, and controls PP1γ-dependent essential DDR regulationCDCA2 regulates the DNA damage response(DDR) in cell cycle. In addition, CDCA2 modulates phosphorylation of major mitotic histone H3 in a PP1-dependent manner.

||
New chat Able
正在加载，请稍候...