CDC6 Monoclonal antibody
CDC6 Monoclonal Antibody for WB, IF, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, rat, mouse
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
6F12D2
Cat no : 66021-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, ROS1728 cells, HEK-293T cells, Jurkat cell, Raji cells |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Product Information
66021-1-Ig targets CDC6 in WB, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Reactivity | human, rat, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CDC6 fusion protein Ag17958 相同性解析による交差性が予測される生物種 |
| Full Name | cell division cycle 6 homolog (S. cerevisiae) |
| Calculated molecular weight | 560 aa, 63 kDa |
| Observed molecular weight | 65 kDa |
| GenBank accession number | BC025232 |
| Gene symbol | CDC6 |
| Gene ID (NCBI) | 990 |
| RRID | AB_2881493 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDC6, also named as CDC18L and HsCDC18, belongs to the CDC6/cdc18 family. It is involved in the initiation of DNA replication and functions as a regulator at the early steps of DNA replication. It also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Defects in CDC6 are the cause of Meier-Gorlin syndrome type 5 (MGORS5). MGORS5 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. This antibody is a mouse monoclonal antibody raised against residues near the C terminus of human CDC6.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC6 antibody 66021-1-Ig | Download protocol |
| IF protocol for CDC6 antibody 66021-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
