Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of human brain using 12974-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12974-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12974-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 12974-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 12974-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12974-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12974-1-AP (CDC5L Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12974-1-AP
IP result of anti-CDC5L (IP:12974-1-AP, 4ug; Detection:12974-1-AP 1:500) with mouse brain tissue lysate 1600 ug.
IHC staining of mouse testis using 12974-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 12974-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12974-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12974-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12974-1-AP (CDC5L antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 12974-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CDC5L antibody (12974-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CDC5L Polyclonal antibody

CDC5L Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12974-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

PCDC5RP, KIAA0432, Cell division cycle 5-like protein, CEF1, Cdc5-like protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human brain tissue, HEK-293 cells, HepG2 cells, mouse brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse testis tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below

Product Information

12974-1-AP targets CDC5L in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3578 Product name: Recombinant human CDC5L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 451-803 aa of BC001568 Sequence: NPEDGMADYSDPSYVKQMERESREHLRLGLLGLPAPKNDFEIVLPENAEKELEEREIDDTYIEDAADVDARKQAIRDAERVKEMKRMHKAVQKDLPRPSEVNETILRPLNVEPPLTDLQKSEELIKKEMITMLHYDLLHHPYEPSGNKKGKTVGFGTNNSEHITYLEHNPYEKFSKEELKKAQDVLVQEMEVVKQGMSHGELSSEAYNQVWEECYSQVLYLPGQSRYTRANLASKKDRIESLEKRLEINRGHMTTEAKRAAKMEKKMKILLGGYQSRAMGLMKQLNDLWDQIEQAHLELRTFEELKKHEDSAIPRRLECLKEDVQRQQEREKELQHRYADLLLEKETLKSKF 相同性解析による交差性が予測される生物種
Full Name	CDC5 cell division cycle 5-like (S. pombe)
Calculated molecular weight	802 aa, 92 kDa
Observed molecular weight	100 kDa
GenBank accession number	BC001568
Gene Symbol	CDC5L
Gene ID (NCBI)	988
RRID	AB_2078510
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q99459
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for CDC5L antibody 12974-1-AP	Download protocol
IHC protocol for CDC5L antibody 12974-1-AP	Download protocol
IP protocol for CDC5L antibody 12974-1-AP	Download protocol
WB protocol for CDC5L antibody 12974-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Oncotarget CDC5L drives FAH expression to promote metabolic reprogramming in melanoma. Authors - Zhichao Gu View Article
	WB	J Biol Chem O-GlcNAcylation of Thr12 / Ser56 in short form O-GlcNAc transferase (sOGT) regulates its substrate selectivity. Authors - Li Liu View Article
human	WB	Cancer Med Circ-PGAM1 promotes malignant progression of epithelial ovarian cancer through regulation of the miR-542-3p/CDC5L/PEAK1 pathway. Authors - Chunmei Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human brain using 12974-1-AP

WB analysis of HEK-293 using 12974-1-AP

WB analysis of HepG2 using 12974-1-AP

WB analysis of HEK-293 using 12974-1-AP

WB analysis of HepG2 using 12974-1-AP

WB analysis of mouse brain using 12974-1-AP

IP experiment of mouse brain using 12974-1-AP

IHC staining of mouse testis using 12974-1-AP

IHC staining of mouse testis using 12974-1-AP

IHC staining of mouse brain using 12974-1-AP