Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells, mouse pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
11824-1-AP targets CDC42EP2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2392 Product name: Recombinant human CDC42EP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC022337 Sequence: MSTKVPIYLKRGSRKGKKEKLRDLLSSDMISPPLGDFRHTIHIGSGGGSDMFGDISFLQGKFHLLPGTMVEGPEEDGTFDLPFQFTRTATVCGRELPDGPSPLLKNAISLPVIGGPQALTLPTAQAPPKPPRLHLETPQPSPQEGGSVDIWRIPETGSPNSGLTPESGAEEPFLSNASSLLSLHVDLGPSILDDVLQIMDQDLDSMQIPT 相同性解析による交差性が予測される生物種 |
| Full Name | CDC42 effector protein (Rho GTPase binding) 2 |
| Calculated molecular weight | 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC022337 |
| Gene Symbol | CDC42EP2 |
| Gene ID (NCBI) | 10435 |
| RRID | AB_10793663 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O14613 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CDC42EP2 (CDC42 Effector Protein 2) is a protein-coding gene that belongs to the Borg family of CDC42 effector proteins. The protein it encodes contains a CRIB (Cdc42/Rac interactive-binding) domain, which mediates its interaction with CDC42, a small Rho GTPase that regulates the formation of F-actin-containing structures. Through this binding, CDC42EP2 is thought to play a role in actin filament assembly and cell shape control, and it appears to negatively regulate CDC42 function. The gene is also known by the aliases CEP2 and BORG1, and is located on chromosome 11q12-q13. Its expression is ubiquitous but variable, with notably high levels in the heart and lower levels in the pancreas and liver.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC42EP2 antibody 11824-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |